广西涠洲岛柳珊瑚共附生真菌多样性及其抑菌活性*

文章使用4种分离培养基, 从10个广西涠洲岛柳珊瑚样本中分离可培养真菌, 通过菌落形态观察和内转录间隔区序列系统发育分析, 得到涠洲岛柳珊瑚共附生真菌多样性信息, 并采用96孔板法测定了真菌发酵液乙酸乙酯提取物的抑菌活性和抗生物被膜形成活性。试验结果表明, 从涠洲岛柳珊瑚样本中分离得到191株共附生真菌, 鉴定为26个种。这些真菌均属于子囊菌门, 分布于6个属: 曲霉属(Aspergillus)、青霉属(Penicillium)、链格孢属(Alternaria)、枝孢菌属(Cladosporium)、黑孢霉属(Nigrospora)和Parengyodontium属。曲霉属(Aspergillus)为优势种属, 占总菌株种类的69.23%; 次优属为枝孢菌属(Cladosporium)。抑菌活性筛选发现有11种真菌至少对一种指示菌有抑制活性, 占菌种总数的42.31%; 有7种真菌对表皮葡萄球菌生物被膜形成具有抑制活性, 半数有效浓度(EC₅₀)范围为40.3~155μg·mL^-1, 菌株Penicillium cinnamopurpureum GXIMD00518、Aspergillus carneus GXIMD00519具有抗耐甲氧西林金黄色葡萄球菌生物被膜形成活性, EC₅₀分别为105.4μg·mL^-1和117.4μg·mL^-1。

Diversity and anti-bacteria activity of the gorgonian derived fungi from Weizhou Island of Guangxi Province*

Four media were used to study culturable fungal diversity in 10 gorgonian samples obtained from Weizhou Island, Guangxi Province. Fungi were further identified by colonial morphology observations and molecular biological analyses. Their anti-bacteria and anti-biofilm activities were determined by 96-well microplate assay. A total of 191 fungal strains were isolated from those gorgonians. All of the 191 fungi belonged to the phylum Ascomycota, and were distributed among six genera: Aspergillus, Penicillium, Alternaria, Cladosporium, Nigrospora, and Parengyodontium. Eleven fungal strains exhibited inhibitory effect against the three strains of pathogenic bacteria, which are Staphylococcus epidermidis ATCC12228, Streptococcus agalactiae ATCC51487, and Streptococcus iniae ATCC29178. The strains P. cinnamopurpureum GXIMD00518 and A. carneus GXIMD00519 also showed anti-MRSA biofilm activities. Gorgonians are rich resources of fungi that deserve further exploration.