影响龙须菜果孢子体形成内外因素的初步研究

龙须菜是中国重要的人工栽培海藻,主要用途是琼胶生产原料和鲍鱼饵料。目前龙须菜大规模栽培采用的是人工夹苗的方式,而其孢子采苗的生产方式也得到越来越多的关注。本文将人工培育的由四分孢子萌发长成的一对龙须菜雌、雄配子体进行受精,测试了雌雄配子体藻枝部位、温度、硝酸盐浓度等因素对果孢子体形成过程的影响,结果表明:龙须菜雌配子体藻枝的不同等级分枝表现出不同的生殖力,一级分枝最尖端新生细胞的成熟速度快于其它等级;温度对果孢子体形成有极显著影响(P<0.01),20℃为最适温度;硝酸盐浓度没有显著影响。

A Preliminary Study on the Internal and External Factors Affecting the Formation of Carposporophytes in Gracilariopsis lemaneiformis

Gracilariopsis lemaneiformis is an important cultivated seaweed in China,which is mainly used as the production of agar and abalone bait.At present the method for large-scale cultivation of Gracilaria lemaneiformis is attaching seedlings into the ropes by hands,and the spore seedling production mode has gotten more and more attention.In this article,the effects of several factors such as branches of female and male gametophyte algae,temperature and nitrate concentration on the formation of carposporophytes were tested by fertilization of a pair of female and male gametophytes grown from tetraspores released from one artificially cultivated tetrasporophyte.The results showed that the different generations of branches of the female gametophyte showed different fertility and the new formed cells at the tip of the first generation branches may mature more rapidly than other generations;temperature had an extremely significant effect on the formation of carposporophyte(P<0.01)and 20℃was the optimal temperature;nitrate concentration had no significant affects.