鱼类生长激素的分离、鉴定及其生理功能研究的进展

主要对８０年代末至９０年代中期国际有关鱼类生长激素的分离，鉴定和生理功能研究的进展予以综述，资料表明，蓝鲨，罗非鱼等２１种鱼类的生长激素已被分离，并阐明鱼类ＧＨ一般是在碱性条件下，将脑垂体匀浆，或取其体外培养的分泌液，经凝胶过滤和反相高效液相色谱纯化得到。

ADVANCES IN THE STUDIES ON THE ISOLATION, CHARACTRIZATION AND PHYSIOLOGICAL FUNCTIONS OF FISH GROWTH HORMONE

The advances in the studies on isolation, characterization andphysiological functions of fish growth hormone (GH) were reviewed based on1988-1994 pub1ished data. About twenty-one fish growth hormones, including blueshark, tilapia etc, have been isolated. Fish growth hormones were mostly obtained byhomogenizing the pitultary or using the media of cultured pituitary, then extractingunder alkaline condition (pH=9-10), chromatographing on a Sephadex column andseparating on a reversed phase HPLC column. Usually the bioactivity of fish growthhormone was determined by the feature of growth promotion to juvenile flsh. Therecent studies indicated that fish GH has the main physiological functions of growthpromotion, hypo-osmoregulation, response of stress, modulating insulin-like growthfactor-I (IGF - I) secretion, regulating the gonadal steroidgenesis through pituitary - sexgland axis, stimulating the peripheral conversion of thyroalne (T4) to triiodothyronine(T3) and inhibiting the synthesis of antifreeze protein in winter flounder.