The extraction of pigments from fresh Laminaria japonica |
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Authors: | Liqun Yang Pengcheng Li Shoujin Fan |
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Affiliation: | [1]Shandong Normal University, Jinan 250014, China [2]Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China |
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Abstract: | The pigments in Laminaria japonica was extracted with six organic solvents and analyzed in spectroscopy analysis.The extractions conditions were screened by an orthogonal test and the quantity of extracted pigments was determined spectroscopically.The results show that:(1) among the six organic solvents,acetone was the most effective one for the extraction; (2) the optimum extraction conditions were as follows:the ratio of S/M (solvent volume/ material weight) was 30ml/g; fresh seaweed was extracted 2 times in 2h; (3) the average total content of pigments was 1.85mg/g (calculated with dry L.japonica). |
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Keywords: | fresh Laminaria japonica total pigment extraction orthogonal test |
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