红砂正常和脱水组织中总RNA提取的改进CTAB法

红砂属于柽柳科超旱生小灌木，是荒漠半荒漠地区植被中的优势种。为了研究促分裂原激活的蛋白激酶(MAPK)信号传导通路在这类植物抗旱中的作用，我们尝试了很多RNA提取的方法都没有获得完整的RNA。在脱水组织中由于多糖、多酚以及其他与核酸能结合或共沉淀的化合物的含量升高而更不容易得到高质量的RNA。以CTAB为基础的改进的RNA提取方法，能成功地从少量的红砂植物的正常组织和脱水组织中获得高质量的RNA。这种方法经济且操作方便，提取的RNA纯度和完整性都很好，可用于RT-PCR反应并成功克隆了550 bp 左右的MAPK基因片段。

Isolation of RNA Using Modified Method of CTAB from Dehydrated and Nondehydrated Reaumuria soongorica Tissues

Reaumuria soongorica belongs to the family of the Tamaricaceae,a perennial super-xerophytic semi-shrub.It is a predominant specces in arid and semi-arid areas.In attempts to evaluate the mechanism of mitogen-activated protein kinases(MAPKs) signaling transduction of drought tolerance in these plants,we experienced that many published protocols for nucleic acid isolation failed to yield RNA of sufficient quality for analysis.It is harder in dehydrated tissues because of higher levels of accumulated polysaccharides,RNases and other compounds that bind and/or coprecipitate with RNA.Therefore,we have modified CTAB(hexadecyltrimethylammonium bromide)-based extraction protocols for isolating intact purified RNA from dehydrated and non-dehydrated organs as a routine isolation of high-quality RNA from small amounts of R.soongorica tissues.The methods developed are simple and effective.The RNA was of sufficient quality for use in a RT-PCR reaction that amplified an about 550 bp fragment of the R.soongorica MAPK gene.