The effect of simulated gastrointestinal digestion on shrimp <Emphasis Type="Italic">Penaeus vannamei</Emphasis> allergenicity |
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Authors: | Yongchao Guo Zhenxing Li Hong Lin |
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Institution: | Food Safety Laboratory, Ocean University of China, Qingdao 266003, China |
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Abstract: | Pen a 1 is a major shrimp allergen that induces an allergy. The aim of this study is to assess the effect of simulated gastrointestinal
fluids on the allergenicity of Pen a 1. Purified Pen a 1 from shrimp (Penaeus vannamei) was subjected to digestion in SGF (simulated gastric fluid) and SIF (simulated intestinal fluid) for a set time. The allergenicity
of digestive Pen a 1 was analyzed by immunoblotting and Ci-ELISA, using pool sera from patients with shrimp specific IgE.
The results showed that Pen a 1 exhibited a decrease in allergenicity with increasing digestion time in the SGF and SIF. However,
Pen a l exhibited strong resistance to digestive fluids, and all yielded fragments (33 kD, 23 kD, and 14 kD) showed allergic
activity. Therefore, anti-digestion may be an important factor for Pen a 1 to induce an allergy. |
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Keywords: | Pen a 1 allergenicity SGF SIF digestion |
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