发酵浒苔对珍珠龙胆石斑鱼生长、非特异性免疫及消化酶活性的影响

浒苔经发酵后按添加比例（0%、1%、2%、3%、4%、5%）分别加入到珍珠龙胆石斑鱼（Epinephelus lanceolatus♂×Epinephelus fuscoguttatus♀）基础饲料中配制成6种饲料，用以探讨发酵浒苔对珍珠龙胆石斑鱼生长、肠消化酶活性及血清非特异性免疫力的影响。选用初始平均体质量为（19.16±0.25）g的珍珠龙胆石斑鱼为研究材料，试验周期28 d。结果表明:发酵浒苔能显著影响石斑鱼的增重率（WGR）和特定生长率（SGR）（P<0.05），且随着发酵浒苔添加水平的升高呈现先升高后降低的趋势，在2%组添加量时显著高于对照组。同时，相比于对照组，各添加组饲料系数（FCR）均有下降，在2%组时显著降低（P<0.05）。添加发酵浒苔可以显著提高血清总超氧化物歧化酶（T-SOD）、碱性磷酸酶（AKP）和酸性磷酸酶（ACP）的活性（P<0.05），但对溶菌酶（LZM）和酚氧化酶（PO）活性影响差异不显著（P>0.05）。在添加组中，淀粉酶、脂肪酶以及胰蛋白酶活性均得到了显著提高（P<0.05），添加水平在2%~3%时效果最好。综合发酵浒苔对珍珠龙胆石斑鱼的作用效果可以看出，发酵浒苔可以明显促进其生长，提高非特异性免疫力及肠道中消化酶活力，建议发酵浒苔在饲料中添加量为2%左右。

Effects of fermented Enteromorpha prolifera on growth performance, non-specific immunity and digestive enzyme activity of pearl gentian grouper

To evaluate the effect of fermentation Enteromorpha prolifera on growth performance, digestive enzyme activities and serum non-specific immunity, 6 diets adding 0%, 1%, 2%, 3%, 4%, 5% fermentation E. prolifera were respectively fed to pearl gentian grouper(Epinephelus lanceolatus♂×Epinephelus fuscoguttatus♀) (19.16±0.25) g for 28 d. The results showed that with the increasing of fermentation E. prolifera level,the weight gain ratio(WGR) and the specific growth ratio(SGR) in each group showed a trend of first rising then falling,while the feed conversion ratio(FCR)was on the contrary. The WGR,SGR and FCR showed significantly better than the control group at the content of 2%(P<0.05). Adding fermentation E. prolifera enhanced the activities of serum total Superoxide dismutase (T-SOD), Alkaline phosphatase (AKP) and acid phosphatase (ACP) significantly (P<0.05), but no significant differences were found in the activities of Lysozyme (LZM) and Polyphenol Oxidase (PO) between trials (P>0.05). With the addition of fermentation E. prolifera, the activity of amylase, lipase and trypsin were significantly increased (P<0.05), and the best level is 2%-3%. In conclusion, fermentation E. prolifera can obviously promote the growth performance, improved the activity of non-specific immunity enzyme in the serum and digestion enzyme in the gut, it is recommended the amount adding is about 2%.