| Identification of SNARE proteins in fish-Tilapia Oreochromis niloticus |
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| 引用本文： | HUANG Xiaohang,LAM Patrick P L,LIN Xuezheng,LIU Chenlin,BIAN Ji,GAISANO Herbert. Identification of SNARE proteins in fish-Tilapia Oreochromis niloticus[J]. 海洋学报(英文版), 2007, 26(1): 159-164 |
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| 作者姓名： | HUANG Xiaohang LAM Patrick P L LIN Xuezheng LIU Chenlin BIAN Ji GAISANO Herbert |
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| 作者单位： | Department of Physiology and Medicine University of Toronto Toronto M5S 1A8 Canada，Department of Physiology and Medicine University of Toronto Toronto M5S 1A8 Canada |
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| 基金项目： | 国家高技术研究发展计划(863计划) , 国家自然科学基金 , The Canadian Institute for Health Research |
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| 摘 要： | 1 IntroductionSecretion is a basic function of all living organ-isms. In fish, like in mammals, neurotransmitters,hormones, enzymes and other macromolecules aresecreted in several neuronal, neuroendocrine and ex-ocrine organs and tissues located in brain,…
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| 关 键 词： | 罗非鱼 SNARE蛋白 分泌 鉴定 |
| 收稿时间： | 2006-01-05 |
| 修稿时间： | 2006-05-08 |
Identification of SNARE proteins in fish-Tilapia Oreochromis niloticus |
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| HUANG Xiaohang,LAM Patrick P L,LIN Xuezheng,LIU Chenlin,BIAN Ji and GAISANO Herbert. Identification of SNARE proteins in fish-Tilapia Oreochromis niloticus[J]. Acta Oceanologica Sinica, 2007, 26(1): 159-164 |
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| Authors: | HUANG Xiaohang LAM Patrick P L LIN Xuezheng LIU Chenlin BIAN Ji GAISANO Herbert |
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| Affiliation: | 1.Key Laboratory of Marine Bioactive Substances, First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China2.Department of Physiology and Medicine, University of Toronto, Toronto, M5S 1A8, Canada |
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| Abstract: | SNARE proteins are a group of membrane-associated proteins involved in exocytosis, secretion and membrane trafficking events in eukaryotic cells. Research on SNARE protein biology has become a more attractive field in recent years, which is applied to marine biology specifically to the fish Tilapia (Oreochromis niloticus). Plasma membrane fractions of different tissues of Tilapia, including brain, liver-pancreas, intestine, skin and muscle, were extracted, and immuno-decorated with isoform-specific antibodies to the SNARE families and associated proteins. The presence of Syntaxins -1A, 2 and 3, SNAP-23 and SNAP-25, VAMP-2, Munc-18-1 and Munc-13 in the brain was identified, which were differentially distributed in the other organ tissues of the fish Tilapia. The distinct distribution of SNARE and associated proteins will serve as the basis for further investigation into their special secretory function in these tissues of the fish. |
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| Keywords: | SNARE proteins exocytosis secretion fish |
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