| An improved PCR method for direct identification of Porphyra （Bangiales, Rhodophyta） using conchocelis based on a RUBISCO intergenic spacer |
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| 引用本文： | 王超,董栋,王广策,张宝玉,彭光,许璞,汤晓荣.An improved PCR method for direct identification of Porphyra （Bangiales, Rhodophyta） using conchocelis based on a RUBISCO intergenic spacer[J].中国海洋与湖沼学报,2009,27(3):513-518. |
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| 作者姓名： | 王超 董栋 王广策 张宝玉 彭光 许璞 汤晓荣 |
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| 作者单位： | WANG Chao,DONG Dong(Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Graduate University of Chinese Academy of Sciences, Beijing 100049, China);WANG Guangce(Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Tianjin University of Science and Technology, Tianjin 300222, China);ZHANG Baoyu,PENG Guang(Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China);XU Pu(Changshu Institute of Technology, Changshu 215500, China);TANG Xiaorong(Ocean University of China, Qingdao 266071, China) |
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| 基金项目： | the National High Technology Research and Development Program of China (863 Program)，Project for Supporting National Development，the National Natural Science Foundation of China，the Natural Science Foundation of Qingdao，the Knowledge Innovation Program of the Chinese Academy of Sciences |
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| 摘 要： |
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| 关 键 词： | Rubisco PCR方法 条斑紫菜 间隔区 丝状体 红藻门 基础 DNA提取方法 |
| 收稿时间： | 25 March 2007 |
An improved PCR method for direct identification of <Emphasis Type="Italic">Porphyra</Emphasis> (Bangiales,Rhodophyta) using conchocelis based on a RUBISCO intergenic spacer |
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| Chao?Wang,Dong?Dong,Guangce?Wang,Baoyu?Zhang,Guang?Peng,Pu?Xu,Xiaorong?Tang.An improved PCR method for direct identification of Porphyra (Bangiales,Rhodophyta) using conchocelis based on a RUBISCO intergenic spacer[J].Chinese Journal of Oceanology and Limnology,2009,27(3):513-518. |
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| Authors: | Chao Wang Dong Dong Guangce Wang Baoyu Zhang Guang Peng Pu Xu Xiaorong Tang |
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| Institution: | [1]Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071 China [2]Graduate University of Chinese Academy of Sciences, Beijing 100049, China [3]Tianjin University of Science and Technology, Tianjin 300222, China [4]Changshu Institute of Technology, Changshu 215500, China [5]Ocean University of China, Qingdao 266071, China |
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| Abstract: | An improved method of PCR in which the small segment of conchocelis is amplified directly without DNA extraction was used to amplify a RUBISCO intergenic spacer DNA fragment from nine species of red algal genus Porphyra(Bangiales,Rhodophyta),including Porphyra yezoensis(Jiangsu,China),P.haitanensis(Fujian,China),P.oligospermatangia(Qingdao,China),P.katadai(Qingdao,China),P.tenera(Qingdao,China),P.suborboculata(Fujian,China),P.pseudolinearis(Kogendo,Korea),P.linearis(Devon,England),and P.fallax(Seattle,USA).... |
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| Keywords: | Rhodophyta Porphyra RUBISCO intergenic spacer DNA sequence PCR |
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