Structural studies on the oligomers from the polysaccharide ofGracilaria textorii (Rhodophyta) using β-agarase and13C-NMR spectroscopy |
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Authors: | Ji Minghou M Lahaye W Yaphe |
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Institution: | (1) Institute of Oceanology, Academia Sinica, Qingdao;(2) Department of Microbiology and Immunology, McGill University, Montreal, Canada;(3) Present address: INRA-LBTG, B. P. 527, 44026 Nantes Cedex, France |
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Abstract: | The 60% ethanolic extract fromGracilaria textorii (Rhodophyta) was degraded with β-agarase, and certain charged (sulfated) and neutral oligosaccharides were separated by using
DEAE Sephadex A25 and Bio- gel P6, P2 chromatographic techniques. Some of the charged oligomers were verified to be neoagarotetraose-63-sulfate (DP2), neoagarohexaose-63, 65-disulfate (DP3) and neoagarooctaose-63, 65, 67-trisulfate (DP4) by using13C- and1H-NMR spectroscopy. One neutral oligomer was assumed to be a mixture of methylated neoagarotetraoses (DP2) by1H-NMR spectroscopy.
These oligomers assigned by their chemical shifts may be used as the model compounds for the structural investigation of the
agar-type sulfated polysaccharides using the β-agarase degradation method.
Contribution No. 1749 from the Institute of Oceanology, Academia Sinica. |
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