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| Construction of Porphyra yezoensis Pure Line from Protoplasts and Its 18S rDNA Sequence Determination | |
| 作者姓名： | LIU Hongquan YU Wengong * DAI Jixun GONG Qianhong SHI Xiaochong YANG Kunfeng Institute of Marine Drugs and Foods Ocean University of China Qingdao P..R.China College of Marine Life Sciences Ocean University of China Qingdao P..R.China |
| 作者单位： | LIU Hongquan 1)，2)，YU Wengong 1)，*，DAI Jixun 2)，GONG Qianhong 1)，SHI Xiaochong 1)，YANG Kunfeng 2) 1) Institute of Marine Drugs and Foods，Ocean University of China，Qingdao 266003，P..R.China 2) College of Marine Life Sciences，Ocean University of China，Qingdao 266003，P..R.China |
| 摘 要： | 1 Introduction PorphyraisthemainobjectforalgafarmingandplaysaveryimportantroleinChinesemarineindus tries.Recently ,therehasbeenagreatlossinPor phyracultivationduetothedegenerationoftheculti var,sothereisanincreasingdemandforgoodPor phyracultivars .Theprerequisiteofthetraditionalbreed ingandbioengineeringresearchofPorphyraistheconstructionofpurelines .Traditionally ,theclassifi cationofPorphyrawasaccordingtotheirmorphologi calcharacteristics .However ,mostmorphologicalfea turesofPorphyraar… |
Construction of Porphyra yezoensis Pure Line from Protoplasts and Its 18S rDNA Sequence Determination |
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| LIU Hongquan ,,YU Wengong ,*,DAI Jixun ,GONG Qianhong ,SHI Xiaochong ,YANG Kunfeng Institute of Marine Drugs and Foods,Ocean University of China,Qingdao ,P..R.China College of Marine Life Sciences,Ocean University of China,Qingdao ,P..R.China.Construction of Porphyra yezoensis Pure Line from Protoplasts and Its 18S rDNA Sequence Determination[J].Journal of Ocean University of China,2004,3(1). | |
| Authors: | Liu Hongquan YU Wengong Dai Jixun GONG Qianhong SHI Xiaochong YANG Kunfeng |
| Institution: | 1. Institute of Marine Drugs and Foods, Ocean University of China, Qingdao 266003, P.R. China ;College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China 2. Institute of Marine Drugs and Foods, Ocean University of China, Qingdao 266003, P.R. China 3. College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China |
| Abstract: | The wild Porphyra yezoensis collected from the Qingdao coast was used to prepare protoplasts by enzyme digestion. The pure line was constructed by cultivating the protoplasts. The 18S rDNA of the P. yezoensis pure line was cloned and sequenced. Sequence analysis was executed for this sequence and other 22 sequences retrieved from GenBank. A phylogenetic tree was constructed using the neighbor joining method. The results revealed a high diversity of 18S rDNA sequences in genus Porphyra and the considerable variation of 18S rDNA sequences in different strains of the same species P. yezoensis and P. tenera. Significant difference of 18S rDNA sequence was observed between P. yezoensis from Qingdao, China, and the two strains of P. yezoensis from Japan, but the three strains of P. yezoensis formed a stable clade in the phylogenetic tree. These results indicate the possibility of interspecies and intraspecies discrimination of Porphyra using the 18S rDNA sequences. |
| Keywords: | Porphyra yezoensis 18S rDNA sequence analysis phylogenetic tree |
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