| 香港巨牡蛎♀×太平洋牡蛎♂异源三倍体的初步研究 |
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| 引用本文： | 刘剑,王昭萍,孔静,于瑞海,王有廷,王廷军.香港巨牡蛎♀×太平洋牡蛎♂异源三倍体的初步研究[J].海洋湖沼通报,2012(1):33-40. |
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| 作者姓名： | 刘剑 王昭萍 孔静 于瑞海 王有廷 王廷军 |
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| 作者单位： | 中国海洋大学海水养殖教育部重点实验室;蓬莱海益水产有限公司 |
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| 基金项目： | 国家重点基础研究发展计划项目(2010CB126406);国家自然科学基金项目(30771622);国家高技术研究发展规划项目(2006AA10A401)资助 |
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| 摘 要： | 本文以香港巨牡蛎(Crassostrea hongkongensis)和太平洋牡蛎(C.gigas)为研究对象,进行牡蛎异源三倍体研究试验。在水温25℃条件下,香港巨牡蛎剥离卵子于海水中浸泡促熟1.5h后,与太平洋牡蛎精子受精,获得最高卵裂率为(24.40±3.79)%。受精后20min,采用盐度为10的低渗海水处理杂交受精卵25min,可获得68.65%的异源三倍体幼虫,孵化率为12.71%。异源三倍体幼虫的存活率和生长率均未表现出优势。受精后第9天,异源三倍体幼虫的存活率仅为(0.116±0.023)%,平均壳长为(103.50±0.85)(m;对照组的存活率和壳长分别为(42.17±2.74)%和(123.25±8.60)μm。幼虫在附着变态前死亡。
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| 关 键 词： | 牡蛎 异源三倍体 低渗诱导 香港巨牡蛎 太平洋牡蛎 |
PRIMARY STUDY ON ALLOTRIPLOID INDUCTION OF CRASSOSTREA HONGKONGENSIS ♀×C. GIGAS ♂ |
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| LIU Jian,WANG Zhaoping,Kong Jing,YU Ruihai,WANG Youting,and WANG TingJun.PRIMARY STUDY ON ALLOTRIPLOID INDUCTION OF CRASSOSTREA HONGKONGENSIS ♀×C. GIGAS ♂[J].Transaction of Oceanology and Limnology,2012(1):33-40. |
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| Authors: | LIU Jian WANG Zhaoping Kong Jing YU Ruihai WANG Youting and WANG TingJun |
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| Institution: | 1.Key Laboratory of Mariculture,Ministry of Education,Ocean University of China, Qingdao 266003,China;2.Penglai Haiyi Fisheries Company,Yantai 265600,China) |
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| Abstract: | Prior to the allotriploid induction,the feasibility of fertilization between Crassostrea hongkongensis(Lam and Morton,2003) eggs and C.gigas(Thunberg,1973) sperm had been verified before.Results showed that cleavage rate of(24.40±3.79) % of the interspecific hybrid can be received with the eggs immersed in 25℃ seawater for 1.5h.The zygotes treated for 25 min with a hypotonic solution of 10 salinities at 20min after fertilization can yield the highest triploidy rate of 68.65%,and the hatchery rate of 12.71%.No statistical advantages of growth rate and survival rate were found in allotriploid group compared with control group.The survival rate and shell length of allotriploid group were(0.116±0.023)% and(103.50±0.85)μm at the 9th day after insemination.However,the survival rate and shell length of control group were(42.17±2.74)% and(123.25±8.60)μm.All allotriploid hybrid larvae died before metamorphism. |
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| Keywords: | Oyster allotriploid hypotonic treatment Crassostrea hongkongensis Crassostrea gigas |
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