| 带鱼(Trichiurus haumela)鱼卵DNA的提取及其18S rDNA初步分析 |
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| 引用本文： | 张继民,刘霜,赵建民,马兆党,张洪亮.带鱼(Trichiurus haumela)鱼卵DNA的提取及其18S rDNA初步分析[J].海洋通报,2009,28(6). |
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| 作者姓名： | 张继民 刘霜 赵建民 马兆党 张洪亮 |
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| 作者单位： | 1. 国家海洋局海洋溢油鉴别与损害评估技术重点实验室,山东,青岛,266033;国家海洋局北海环境监测中心,山东,青岛,266033
2. 中国科学院海洋研究所,实验生物学重点实验室,山东,青岛,266071 |
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| 基金项目： | 国家海洋局近岸海域生态环境重点实验室基金“应用DNA技术鉴定鱼卵的实验研究”(200609) |
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| 摘 要： | 目前应用显微镜技术很难进行鱼卵种类的准确鉴定,本文尝试采用DNA技术开展鱼卵的鉴定工作。以带鱼(Trichiurus haumela)鱼卵为研究对象,采用苯酚-氯仿法提取了其DNA基因组,并采用PCR及克隆测序的方法,对其18S rDNA序列进行了测定与初步分析。结果表明,提取的带鱼(T.haumela)鱼卵DNA基因组片段长度约23kb,PCR扩增片段长度约1.8kb;带鱼(T.haumela)鱼卵18S rDNA与其它已知鱼类的18S rDNA序列具有一定的差异性,可以用其进行带鱼(T.haumela)鱼卵的初步鉴定。
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| 关 键 词： | 带鱼 鱼卵 18S rDNA |
Preliminary study on DNA extraction and 18S rDNA of the Trichiurus haumela fish egg |
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| ZHANG Ji-min,LIU Shuang,ZHAO Jian-min,MA Zhao-dang,ZHANG Hong-liang.Preliminary study on DNA extraction and 18S rDNA of the Trichiurus haumela fish egg[J].Marine Science Bulletin,2009,28(6). |
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| Authors: | ZHANG Ji-min LIU Shuang ZHAO Jian-min MA Zhao-dang ZHANG Hong-liang |
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| Institution: | 1. Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology;SOA;Qingdao 266003;China;2. North China Sea Environment Monitoring Center;3. Key laboratory of Experimental Marine Bology;Institute of Oceanology of Chinese Academy of Sciences;Qingdao 266071;China |
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| Abstract: | For the moment,it is difficult to distinguish different species of fish eggs with microscopical technique,and that DNA technique was applied to identify species of fish eggs in the paper. The Trichiurus haumela fish eggs was tested and its genome was extracted with the method of phenol-chloroform and its 18S rDNA sequence was also studied with PCR and clone technique. The results showed that the gene sequence length of the Trichiurus haumela fish eggs was about 23 kb,and that the sequence length of PCR prod... |
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| Keywords: | Trichiurus haumela fish egg 18S rDNA |
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