| SERUM IMMUNOGLOBULIN OF THE MANDARIN FISH,SINIPERCA CHUATSI WITH DEVELOPMENT OF POLYCLONAL ANTIBODY |
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| 作者姓名： | 张永安 聂品 |
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| 作者单位： | StateKeyLaboratoryofFreshuwuterEcologyandBiotechnology,andLaboratoryofFishDisenses,InstituteofHydrobiology,ChineseAcademyofSciences,Wuhan430072 |
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| 基金项目： | ProjectNo.3 0 0 2 5 0 3 5supportedbyNSFC，StateKeyLaboratoryofFreshwaterEcologyandBiotechnology (2 0 0 0FB0 2 )，theChineseAcademyofSciences (KZ95 1 1 B1 1 1 1 ) |
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| 摘 要： | Serum immunoglobulin from the mandarin fish,or the so-called Chinese perch,Siniperca chuatsi(Basilewsky),was successfully purified using affinity chromatography.Heavy and light chains were detected on electrophoresis gel,with molecular weights being estimated at 72 and 29 kDa,respectively.The tetrameric IgM of S.chuatsi was calculated to be 808 kDa.The rabbit polyclonal antisera against the purifed immunoglobulin were developed and tested by Westem blot analysis.The antisera reacted strongly with the heavy chains of S.chuatsi immunoglobulin.Humoral immune responses of the mandarin fish can then be examined using the developed polyclonal antibody.
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| 关 键 词： | 免疫球蛋白 中国金鲈 多克隆抗体 体液免疫反应 鱼类 |
SERUM IMMUNOGLOBULIN OF THE MANDARIN FISH, SINIPERCA CHUATSI WITH DEVELOPMENT OF POLYCLONAL ANTIBODY |
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| ZHANG Yong an,NIE Pin.SERUM IMMUNOGLOBULIN OF THE MANDARIN FISH,SINIPERCA CHUATSI WITH DEVELOPMENT OF POLYCLONAL ANTIBODY[J].Chinese Journal of Oceanology and Limnology,2002,20(4):332-337. |
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| Authors: | ZHANG Yong an NIE Pin |
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| Abstract: | Serum immunoglobulin from the mandarin fish, or the so called Chinese perch, Siniperca chuatsi (Basilewsky), was successfully purified using affinity chromatography. Heavy and light chains were detected on electrophoresis gel, with molecular weights being estimated at 72 and 29 kDa, respectively. The tetrameric IgM of S. chuatsi was calculated to be 808 kDa. The rabbit polyclonal antisera against the purifed immunoglobulin were developed and tested by Western blot analysis. The antisera reacted strongly with the heavy chains of S. chuatsi immunoglobulin. Humoral immune responses of the mandarin fish can then be examined using the developed polyclonal antibody. |
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| Keywords: | immunoglobulin polyclonal antibody mandarin fish Chinese perch Siniperca chuatsi |
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