应用新一代测序技术测定大室别藻苔虫线粒体基因组全序列

线粒体基因组的获得传统上通常是采取长PCR结合鸟枪法或步移法测序。近年来新一代测序技术蓬勃发展,以454,Solexa和SOLiD测序平台为代表。应用新一代高通量测序技术(Solexa)并结合巢式PCR扩增,完成了大室别藻苔虫(Membranipora grandicella)的线粒体基因组全序列。大室别藻苔虫线粒体基因组是目前国际上获得的第一条苔藓动物软壁亚目线粒体基因组全序列,基因组全长为15861 bp,比已完成的4种苔藓动物线粒体基因组略大。大室别藻苔虫线粒体基因组包含13个蛋白质编码基因、2个核糖体RNA基因和20个转运RNA基因。通过与已测得的苔藓动物进行比较,发现目前已完成的5个苔藓动物线粒体基因组的基因排列顺序显著不同。苔藓动物线粒体基因组基因排列的比较,今后可能会成为探讨该类群系统演化关系的重要信息来源。

Determination of Membranipora grandicella complete mitochondrial genome sequence based on next-generation sequencing technology

Traditionally, the mitochondrial genome sequences were usually obtained by shotgun or primers-walking combination with long PCR. The next-generation sequencing technologies are developing fastly in recent years, such as 454, Solexa and SOLiD sequencing platform. Membranipora grandicella complete mitochondrial genome sequence was determined based on next-generation high-throughput sequencing technology (Solexa) and nested PCR amplification. The complete mitochondrial genome of M. grandicella is 15,861 bp in length, which is the first representative from the suborder Malacostegina (Bryozoa), and is relatively larger when compared with mtDNAs of other study bryozoans. The mitochondrial genome contains 13 protein-coding genes, two ribosomal RNAs and 20 transfer RNAs. Compared with other study bryozoans, gene arrangements of five bryozoans mitochondrial genomes are significantly different. Comparison of bryozoans mitochondrial gene arrangements can be important information sources for exploring the phylogenetic relationships within the group.