| 低温选择大黄鱼(Pseudosciaena crocea)的肝脏蛋白质组双向电泳分析 |
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| 引用本文： | 李明云,吴玉珍,冀德伟,吴海庆,陈 炯,史雨红,罗海忠,柳敏海,傅荣兵.低温选择大黄鱼(Pseudosciaena crocea)的肝脏蛋白质组双向电泳分析[J].海洋与湖沼,2010,41(3):348-351. |
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| 作者姓名： | 李明云 吴玉珍 冀德伟 吴海庆 陈 炯 史雨红 罗海忠 柳敏海 傅荣兵 |
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| 作者单位： | 1. 应用海洋生物技术教育部重点实验室,宁波大学,宁波,315211
2. 浙江省舟山市水产研究所,舟山,316000 |
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| 基金项目： | 国家高技术研究发展计划(863)项目, 2006AA10A405 号; 浙江省重大项目, 2006C12005 号; 宁波市重大项目, 2008C10022号; 长江学者和创新团队发展计划项目, IRT0734 号。 |
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| 摘 要： | 采用双向电泳技术,对低温选择组与对照组大黄鱼进行肝脏蛋白质组双向电泳分析。结果表明,低温选择组肝脏蛋白点1673±47个,对照组1651±43个,共有19个蛋白点经低温选择后,表达量显著变化。从中挑选3个差异蛋白点进行肽指纹图谱(MALDI-TOF-MS)或串联质谱(LC-MS-MS)分析,然后MS-BLAST数据库搜寻。低温选择组大黄鱼肝脏MHC和CFL2蛋白表达显著上调,而2-CysPrxs蛋白表达显著下调。说明低温选育组大黄鱼机体抗细胞凋亡能力明显增强,预示低温选择后留下的大黄鱼在低温环境中生存能力更强。
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| 关 键 词： | 大黄鱼 低温选育 肝脏 双向电泳 |
| 收稿时间： | 2009/9/25 0:00:00 |
| 修稿时间： | 2009/11/23 0:00:00 |
2-DE ANALYSIS OF LIVER OF PSEUDOSCIAENA CROCEA AFTER LOW TEMPERATURE BREEDING |
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| LI Ming-Yun,WU Yu-Zhen,JI De-Wei,WU Hai-Qing,CHEN Jiong,SHI Yu-Hong,LUO Hai-Zhong,LIU Min-Hai and FU Rong-Bing.2-DE ANALYSIS OF LIVER OF PSEUDOSCIAENA CROCEA AFTER LOW TEMPERATURE BREEDING[J].Oceanologia Et Limnologia Sinica,2010,41(3):348-351. |
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| Authors: | LI Ming-Yun WU Yu-Zhen JI De-Wei WU Hai-Qing CHEN Jiong SHI Yu-Hong LUO Hai-Zhong LIU Min-Hai and FU Rong-Bing |
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| Institution: | Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University;Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University;Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University;Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University;Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University;Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University;Fisheries Institute of Zhoushan of Zhejiang Province;Fisheries Institute of Zhoushan of Zhejiang Province;Fisheries Institute of Zhoushan of Zhejiang Province |
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| Abstract: | We used 2-DE to compare the liver proteomes of Pseudosciaena crocea with or without low-temperature breeding, and found similar number of proteins expressed in the low-temperature breeding group (1673±47) and the control group (1651±43). The expressions of 19 proteins were significantly altered after low temperature breeding. Three proteins (MHC, CFL2 and 2-Cys Prxs) were identified by MALDI-TOF-MS/LC-MS-MS and MS-BLAST searching. The expressions of MHC and CFL2 protein were up-regulated remarkably, whereas the expression of 2-Cys Prxs was down-regulated remarkably, suggesting the anti-apoptosis ability of the low-temperature breeding group is strengthed. |
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| Keywords: | Pseudosciaena crocea Low temperature breeding Liver Two-dimentionlal gel electrophoresis(2-DE) |
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