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RAPD标记鉴别红鳍东方鲀和假睛东方鲀种群的初步研究
引用本文:刘振辉,石拓,柳学周,陈超,孔杰. RAPD标记鉴别红鳍东方鲀和假睛东方鲀种群的初步研究[J]. 海洋湖沼通报, 1999, 0(4)
作者姓名:刘振辉  石拓  柳学周  陈超  孔杰
作者单位:青岛海洋大学水产学院!266003 (刘振辉),黄海水产研究所育种室!266071 (石拓,柳学周,陈超),黄海水产研究所育种室!266071(孔杰)
基金项目:“948”办公室红鳍东方鲀纯种引进项目
摘    要:用RAPD技术对东方纯属(Fugu)的红鳍东方鲀(Fugu rubripes)和假睛东方鲀(Fugupseudommus)共三个群体进行分析,经20个随机引物扩增,得到每一个体的多态片段。比较所有图谱,没有发现能够鉴别三个群体的特异性谱带;而且采自日本的红鳍东方纯与采自中国的红鳍东方鲀的电泳图谱基本相似。结果还表明,红鳍东方纯与假睛东方鲀电泳图谱的差异也甚小,说明二者的亲缘关系非常接近,这与以前用同工酶进行分析的结果相一致(王可玲等,1984),

关 键 词:RAPD标记  鉴别  红鳍东方  假睛东方

IDENTIFICATION OF THREE POPULATIONS (CHINESE FUGU RUBRIPES, JAPANESE FUGU RUBRIPES AND FUGU PSEUDOMMUS) USING RAPD MARKERS
LIU Zhen-hui. IDENTIFICATION OF THREE POPULATIONS (CHINESE FUGU RUBRIPES, JAPANESE FUGU RUBRIPES AND FUGU PSEUDOMMUS) USING RAPD MARKERS[J]. Transaction of Oceanology and Limnology, 1999, 0(4)
Authors:LIU Zhen-hui
Abstract:Three populations including Fugu rubripes domesticated in China, Fagu rubripes domesticated in Japan and natural Fugu pseudommus in Qingdao were analyzed using the RAPD technology and the pattern of each sample was obtained. Altogether 20 primers were used in this experiment. No markers were found to distinguish them in the study and the patterns of Fugu rubripes from Japan and Fugu rubripes from China are almost the same. At the same time, the difference of the patterns between Fugu rubripes and Fugu pseudommus is very small. It shows that the affinities between them are very near.
Keywords:RAPD marker   population identification   Fugu rubripes    Fugu pseudommus
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