The Accumulation of Crimidin in Some Fish Organs

The accumulation of crimidin in total samples and in some organs of five fish species was studied under experimental conditions. The species were Cyprinus carpio, Carassius carassius, Tinca tinca, Scardinus erythrophthalmus and Leucaspius delineatus. Fish were exposed to concentrations of 10 mg · l^?l and 50 mg · l^?1. During short-term experiments water and fish samples were taken at intervals of t = 0, 6, 12, 24, 48 and 72 hours, during the long-term experiment sampling was performed weekly for six weeks. Total samples were analysed for C. carassius, T. tinca, S. erythrophthalmus and L. delineatus. Samples of individual organs and tissues were taken as follows: C. carpio – gills, digestive tract, muscular tissue, kidneys, gonads; C. carassius – gills, digestive tract, muscular tissue, ovaries, testes. Crimidin was determined by gas chromatography. In samples from the short-term experiments at a concentration of 10 mg · l^?1 in the water the amount was roughly 10¹–10 mg · kg^?1, at a concentration of 50 mg · l^?1 in the water roughly 10¹ mg · kg^?1. In most cases the accumulation coefficient was lower than 1.0. The accumulation capacity of individual fish species did not differ greatly. Of the internal organs only the kidneys had a high accumulation capacity, otherwise the highest values were found in muscular tissue and the gills. After one – three weeks the amount of crimidin in most organs falls, and after transfer to clean water there is a general sharp decline. Thus crimidin is not firmly bound in the body.