For the simulation of the transport of dissolved matter particle models can be used. In this paper a technique is developed for the identification of uncertain parameters in these models. This model calibration is formulated as an optimization problem and is solved with a gradient based algorithm. Here adjoint particle tracks are used for the calculation of the gradient of the cost function. The performance of the calibration method is illustrated by simulations and an application to a river Rhine water quality calamity in November 1986.