水流速率对全缘马尾藻幼孢子体生长和生理活性的影响

在0、0.5、1、2、4、8 cm/s 等5 种不同水流速率下培养全缘马尾藻（Sargassum integerrimum）幼孢子体,研究水流速率对幼孢子体质量、叶绿素a 含量、SOD 活力、CAT 活力和蛋白质浓度等生理指标的影响.结果表明,以1、2、4 cm/s 流速的幼孢子体质量增长最快,3 种流速的增长差异不具统计学意义（P〉0.05）.在生理指标方面：叶绿素a 含量以1、2、4 cm/s 流速最高,3 种流速的含量差异不具统计学意义（P〉0.05）;SOD 比活性以流速0.5、1、2 cm/s 的活力最低,各组间差异不具统计学意义（P〉0.05）;CAT 比活性以流速1、2 cm/s 的活力最低,组间差异不具统计学意义（P〉0.05）;蛋白质含量以1、2、4 cm/s 流速最大,组间差异具统计学意义（P〈0.05）.水流速率对体质量增长和各生化指标均产生显著的影响,以流速1～4 cm/s 培育全缘马尾藻培幼孢子体较合适.

The Effects of Water Velocity on the Growth and Physiological Activitiesof Juvenile Sporophyte of Sargassum integerrimum

In the laboratory, the effects of different water velocities （0,0.5,1,2,4,8 cm/s） on thephysiological indexes including the mass, the content of chlorophyll a ,the activity of SOD and CAT andthe protein concentration of the Juvenile sporophyte of Sargassum integerrimum were studied aiming atproving references for the seeding production. .The results indicated that the effects of the water velocityon the ponderal growth and the biochemical indexes were significant. The mass grow fastest under theconditions of water velocities of 1 cm/s, 2 cm/s and 4 cm/s, with no significant difference among them.In terms of physiological indexes,the highest content of chlorophyll appeared when the water velocitiesare 1 cm/s、2 cm/s and 4 cm/s, with no significant difference among them. The lowest specific activity ofSOD appeared when water velocities are 0.5 cm/s,1 cm/s, 2 cm/s , with no significant differences amongthem. The lowest specific activity of CAT appeared at the conditions of water velocities of 1 cm/s,2cm/s , with no significant differences among them. The highest content of protein appeared at theconditions of water velocities of 1 cm/s, 2 cm/s, 4 cm/s , with no significant differences amongthem（P〈0.05）. On the whole, in the speed ranges, the indexes all changed regularly with the increasingof water velocity, and in terms of juvenile sporophyte culture, the ranges of water velocity from 1 cm/s to 4cm/s are more appropriate.