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斜带髭鲷养殖群体遗传多样性的同工酶研究
引用本文:梁君荣,王军,苏永全,蔡岩,王德祥. 斜带髭鲷养殖群体遗传多样性的同工酶研究[J]. 台湾海峡, 2003, 22(1): 19-23
作者姓名:梁君荣  王军  苏永全  蔡岩  王德祥
作者单位:厦门大学海洋系、亚热带海洋研究所,福建,厦门,361005
基金项目:国家自然科学基金资助项目 (30 0 70 5 95 ),福建省自然科学基金资助项目 (B80 0 10 0 0 3)
摘    要:应用聚丙烯酰胺凝胶电泳(PAGE)技术,对取自广东饶平海水网箱养殖的斜带髭鲷(Hapalogenys nitens)人工苗养殖群体同工酶的多态性进行分析。结果表明:在对48尾鱼所检测出的11种同工酶的23个基因座位中,超氧化物歧化酶(SOD)、苹果酸脱氢酶(MDH)过氧化物酶(PER)等酶的基因座位上发现多态性,其平均多态位点比例(P)为17.39%,基因座位有效等位基因的平均数(Ne)为1.174,平均杂合度的观测值(Ho)为0.051,预期值(He)为0.039,Hardy-Weinberg遗传偏离指数(D)为0.291。这说明该养殖群体具有较高的遗传变异水平。

关 键 词:斜带髭鲷 遗传多样性 同工酶 聚丙烯酰胺凝胶电泳 网箱养殖
文章编号:1000-8160(2003)01-0019-05

Isoenzyme analysis of genetic diversity in cultured Hapalogenys nitens stock
LIANG Jun-rong,WANG Jun,SU Yong-quan,CAI Yan,WANG De-xiang. Isoenzyme analysis of genetic diversity in cultured Hapalogenys nitens stock[J]. Journal of Oceanography In Taiwan Strait, 2003, 22(1): 19-23
Authors:LIANG Jun-rong  WANG Jun  SU Yong-quan  CAI Yan  WANG De-xiang
Abstract:Isoenzymes isolated from cultured Hapalogenys nitens stock collected from Raoping in Guangdong in January 2001 were investigated by polyacrylamid gel electrophoresis (PAGE) in this paper. Among the 23 loci in 11 enzymes examined, three loci polymorphic were found. The mean level of polymophism is 17.39% and heterozygosity is 0.051. Isoenzymes analysis values of H.nitens are mediate within the range of the values reported for other marine fish populations. And compared with other cultured fish populations such as Nibea miichthioides and Pseudosciaena crocea, H.nitens shows higher genetic diversity.
Keywords:Hapalogenys nitens  genetic diversity  isoenzyme  PAGE
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