Effects of virus infection on expression of cell cycle regulatory proteins in the unicellular marine algae Emiliania huxleyi |
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Authors: | LIU Jingwen Gunnar Bratbak ZHENG Tianling and Runar Thyrhaug |
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Institution: | 1.Bioengineering College of Jimei University, Xiamen 361021, China2.Department of Microbiology, University of Bergen, Bergen N-5020, Norway3.Key Laboratory of MOE for Coast and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, China;State Key Laboratory of Marine Environmental Sciences of Xiamen University, Xiamen 361005, China |
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Abstract: | The authors have investigated the biochemical events by which marine algal virus infection induces cell cycle arrest. The key G2/M-phase regulatory proteins are analyzed by immunobloting in unicel-lular Emiliania huxleyi, suggesting that virus induced cell cycle arrest is related with virus's effect on cyclins and cyclin dependent kinases. E. huxleyi virus (EhV) represses Cdc2/cyclinB complex activity by inhibiting the activity of Cdc2 kinase in a phosphorylation-related manner, blocking host cells G2/M checkpoint. Dephosphorylated/inactive Cdc25C combined with up-regulation of Wee1 expression at early infect period appears to be important mechanisms by which EhV represses Cdc2/cyclinB complex activity that is required for entry into M phase. This study has allowed us to confirm that algal virus infection leads to selective activation or inhibition of certain cell-cycle factors, which may play a significant role in establishing a more efficient environment for viral gene expression and DNA replication. |
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Keywords: | marine algal virus cell cycle regulators expression Emiliania huxleyi |
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