| 马粪海胆的染色体制备和染色体数目 |
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| 引用本文： | 董宝贤,林泰禧.马粪海胆的染色体制备和染色体数目[J].中国海洋大学学报(自然科学版),1991(3). |
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| 作者姓名： | 董宝贤 林泰禧 |
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| 作者单位： | 青岛海洋大学生物系
(董宝贤)，青岛海洋大学图书馆(林泰禧) |
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| 摘 要： | 以马粪海胆胚胎为材料,提出染色体制备方法。并指出马粪海胆染色体数目为2n=46
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| 关 键 词： | 马粪海胆 染色体数目 胚胎细胞 二倍体 |
THE PREPARATION AND NUMBER OF CHROMOSOMES OF HEMICENTROTUS PULCHERRIMUS |
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| Dong Baoxian.THE PREPARATION AND NUMBER OF CHROMOSOMES OF HEMICENTROTUS PULCHERRIMUS[J].Periodical of Ocean University of China,1991(3). |
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| Authors: | Dong Baoxian |
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| Institution: | Dong Baoxian(Department of Biology)Lin Taixi(Library) |
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| Abstract: | The chromosomes of sea urchin are studied for breeding.Hemicentrotus pulcherriis used as experimental material in this paper.Method of the chromosomes preparation: Eggs were fertilized artificially with sperms.Early embryos were treated with colchicin and Ca-free sea water.Dissociated cells were swollen with hypotonic KCl (0.075M) The cells were fixed by mcthanol-acetic acid fixative,dried in air The chromosomes stained with Giemsa.Chromosomes numbers were analysed The 2n chromosomes number of H.pulcheri-imus have been concluded to be 46. |
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| Keywords: | Hemicentrotus pulcherrimus chromosome number embryo cells diploid |
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