Phytolith indices as proxies of grass subfamilies on East African tropical mountains

The main objective of this paper is to provide researchers that investigate fossil phytolith assemblages and model/data comparisons a new tool for estimating C₃/C₄ grass composition over time. We tested the reliability of modern soil phytolith assemblages and phytolith indices for tracing the dominance of different grass subfamilies and tree cover density. We analyzed modern soil phytolith assemblages from sites over elevation gradients on Mount Kenya (Kenya), Mount Rungwe and around Lake Masoko (southern Tanzania). These data were compared with available botanical data. A phytolith index named Ic, proved to be an effective proxy of the proportions of Pooideae, Arundinoideae and Bambusoideae grasses (mainly C₃ grasses) versus Panicoideae grasses (mainly C₄ grasses), increasing with elevation in East-Africa. When tropical mountains are covered by open habitats (e.g. grasses and shrublands), Ic should be a reliable proxy of the C₃/C₄ grass composition. These results highlight the value of the phytolith index Ic, when interpreting paleo-environmental records from tropical mountains, to: 1) better understand past local and regional C₃/C₄ grass distributions and associated climatic changes and 2) increase the set of C₃/C₄ data available for model/data comparisons.