Phytoplankton diversity in the East China Sea and Yellow Sea measured by PCR-DGGE and its relationships with environmental factors |
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Authors: | Jing Sun Zhigang Yu Yahui Gao Qianqian Zhou Yu Zhen Hongtao Chen Liyuan Zhao Qingzhen Yao Tiezhu Mi |
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Institution: | (1) Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, People’s Republic of China;(2) Graduate School of the Chinese Academy of Sciences, Beijing, 100039, People’s Republic of China; |
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Abstract: | Relationships between phytoplankton community composition and environmental variables in the East China Sea (ECS) and Yellow
Sea (YS) were investigated using geochemical and molecular microbiology methods. The diversity of phytoplankton was characterized
using cultivation-independent PCR-based denaturing gradient gel electrophoresis (DGGE). Groups resulting from unweighted pair-group
method with arithmetic averages clustering of the DGGE profiles showed good consistency with the eco-environmental characteristics
of the sea area they belonged to. Additionally, the clustering results based on DGGE fingerprinting and those based on morphological
compositions were practically identical. The relationship of phytoplankton diversity to environmental factors was statistically
analyzed. Temperature, dissolved inorganic nitrogen (DIN), and silicate-Si were found significantly related to the phytoplankton
community composition. Canonical correspondence analysis (CCA) was performed to reveal the relationship between community
composition and these three environmental factors. Generally, values of the ECS are clearly separated from those of the YS
in the CCA biplot, due to mainly the effect of temperature and DIN. |
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