| 西施舌同工酶组织特异性研究 |
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| 引用本文： | 程汉良,孟学平,董志国. 西施舌同工酶组织特异性研究[J]. 海洋湖沼通报, 2006, 0(2): 44-51 |
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| 作者姓名： | 程汉良 孟学平 董志国 |
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| 作者单位： | 1. 淮海工学院,江苏省海洋生物重点建设实验室,连云港,222005;南京农业大学无锡渔业学院,无锡,214081
2. 淮海工学院,江苏省海洋生物重点建设实验室,连云港,222005 |
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| 基金项目： | 江苏省海洋生物技术重点建设实验室开放课题(2005HS009)，淮海工学院引进人才科研启动基金(KK01050)资助 |
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| 摘 要： | 采用不连续垂直板聚丙烯酰胺凝胶电泳技术,对西施舌5种组织(肝胰脏、鳃、后闭壳肌、外套膜和足)的酯酶(EST)、苹果酸脱氢酶(MDH)和超氧化物歧化酶(SOD)同工酶进行了研究。同工酶图谱显示:西施舌上述组织的3种同工酶具有一定的组织特异性。其中,肝和鳃组织EST酶活性较高,闭壳肌MDH酶活性最高,而肝组织SOD酶活性略高,上述组织是进行同工酶分析的理想材料。
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| 关 键 词： | 西施舌 同工酶 组织特异性 |
| 文章编号： | 1003-6482（2006）02-0044-08 |
| 收稿时间： | 2005-08-05 |
| 修稿时间： | 2005-08-05 |
STUDY ON THE TISSUE SPECIFICITY OF ISOZYMES INCOELOMACTRA ANTIQUATE SPENGLER |
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| CHENG Hanliang,MENG Xueping,DONG Zhiguo. STUDY ON THE TISSUE SPECIFICITY OF ISOZYMES INCOELOMACTRA ANTIQUATE SPENGLER[J]. Transaction of Oceanology and Limnology, 2006, 0(2): 44-51 |
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| Authors: | CHENG Hanliang MENG Xueping DONG Zhiguo |
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| Affiliation: | 1. Huaihai Institute of Technology, Key Constructing Lab of Marine Biotechnology of Jiangsu Province, Lianyungang 222005 ; 2. Fisheries College, Nanjing Agriculture University, Wuxi 214081, P. R. China |
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| Abstract: | Three isozymes(EST,MDH,SOD) from five different tissues(liver,gill,posterior adductor muscle,mantle and foot) of Coelomactra antiquate were analyzed by vertical polyacrylamide gel electrophoresis(PAGE).The results showed that there was a slight diversity in the EST,MDH and SOD isozyme phenotypes among the five tissues.The activitys of EST in liver and gill,MDH in adductor muscle and SOD in liver were higher than in other tissues.These tissues are better materials for isozyme analysis. |
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| Keywords: | Coelomactra antiquate Spengler isozyme tissue specificity |
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