| 青岛、威海水域夏冬季表层沉积物细菌多样性的初步研究 |
| |
| 引用本文： | 肖慧,张艳,张喆,朱琳,游偲,唐学玺.青岛、威海水域夏冬季表层沉积物细菌多样性的初步研究[J].中国海洋大学学报(自然科学版),2009,39(4). |
| |
| 作者姓名： | 肖慧 张艳 张喆 朱琳 游偲 唐学玺 |
| |
| 作者单位： | 1. 中国海洋大学海洋生命学院,山东,青岛,266003
2. 中国水产科学研究院黄海水产研究所,山东,青岛,266071;中国海洋大学海洋生命学院,山东,青岛,266003 |
| |
| 基金项目： | 山东省我国近海海洋综合调查与评价(908计划)，教育部新世纪优秀人才支持计划，国家高技术研究发展计划(863计划)，公益性行业(农业)科研专项经费子课题 |
| |
| 摘 要： | 以青岛、威海水域夏冬两季表层沉积物中的基因组DNA为模板,扩增沉积物样品中细菌的16S rDNA 片段,并构建其相应文库.进行16S rDNA序列分析,并与数据库中的序列进行比对.分析结果表明:2个站位沉积物样品细菌分别属于11个主要细菌门类,包括:变形菌门(Proteobacteria)、浮霉菌门(Plancomycene)、芽单胞菌门(Gemmatimonadetes)、酸杆菌门(Acidobacteria)、硝化螺菌门(Nitrospirae)、疣微菌门(Verrucomicrobial)、拟杆菌门(Bacteroidetes)、绿屈挠菌门(Chloroflexi)、厚壁菌门(Firmicutes)、高G+C含量革兰氏阳性菌放线菌门(Actinobacteria)和低G+C含量革兰氏阳性菌梭菌门(Clostridiales).其中变形菌门是主要类群,且γ-变形菌纲(γ-Proteobacteria)占优势.与QD2站位相比,QD1站位沉积物细菌多样性随季节变化不大.
|
| 关 键 词： | 沉积物 细菌 多样性 青岛 威海 |
A Preliminary Study on the Bacterial Diversity in Surface Sediments from the Coastal Water of Qingdao and Weihai in Summer and Winter |
| |
| XIAO Hui,ZHANG Yan,ZHANG Zhe,ZHU Lin,YOU Cai,TANG Xue-Xi.A Preliminary Study on the Bacterial Diversity in Surface Sediments from the Coastal Water of Qingdao and Weihai in Summer and Winter[J].Periodical of Ocean University of China,2009,39(4). |
| |
| Authors: | XIAO Hui ZHANG Yan ZHANG Zhe ZHU Lin YOU Cai TANG Xue-Xi |
| |
| Institution: | 1.College of Marine Life Science;Ocean University of China;Qingdao 266003;China;2.Department of Mariculture Ecology and Carrying Capacity;Yellows Sea Fisheries Research Institute;Qingdao 266071;China |
| |
| Abstract: | Based on the purified DNA extracted from surface sediment samples from coastal water of Qingdao and Weihai in summer and winter,16S rDNA fragments of bacteria were PCR amplified and 16S rDNA clone libraries were constructed.Sequence analysis of 16S rDNA,compared with the published sequences in Genbank database and guided by the phylogenetic tree,the bacteria fell into eleven main lineages: Proteobacteria,Plancomycene,Gemmatimonadetes,Acidobacteria,Nitrospirae,Verrucomicrobial,Bacteroidetes,Chloroflexi,Firmi... |
| |
| Keywords: | 16S rDNA sediment 16S rDNA bacterial diversity Qingdao Weihai |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
