| 卵形鲳鲹3个养殖群体的微卫星多态性分析 |
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| 引用本文： | 吉磊,区又君,李加儿. 卵形鲳鲹3个养殖群体的微卫星多态性分析[J]. 热带海洋学报, 2011, 0(3) |
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| 作者姓名： | 吉磊 区又君 李加儿 |
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| 作者单位： | 中国水产科学研究院南海水产研究所;上海海洋大学水产与生命学院; |
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| 基金项目： | 国家高技术研究发展计划重点项目(2006AA10A414); 中央级公益性科研院所基本科研业务费专项资金项目(2008YD02) |
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| 摘 要： | 运用6对微卫星引物分别对海南、深圳、福建3个地区的卵形鲳鲹Trachinotus ovatus养殖群体的遗传差异进行分析。结果表明,3个养殖群体的平均等位基因数(Na)为3.67—3.83,平均有效等位基因数(Ne)为2.43—3.03,平均观测杂合度(Ho)为0.48—0.66,平均期望杂合度(He)为0.56—0.64,平均多态信息含量(PIC)为0.49—0.55,可见3个养殖群体的遗传多样性较高。Hardy-weinberg平衡遗传偏离指数(d)显示海南和深圳养殖群体杂合子过剩(分别为0.10和0.12),而福建养殖群体则出现杂合子缺失(-0.08)。统计检验发现3个群体中各位点的遗传偏离并不显著(p>0.05)。3个养殖群体的遗传相似度及遗传距离信息聚类分析结果表明,福建与深圳养殖群体的亲缘关系较近,两者与海南养殖群体的亲缘关系较远。3个养殖群体的确切来源还有待进一步研究。
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| 关 键 词： | 卵形鲳鲹 养殖群体 微卫星DNA 遗传多样性 |
Genetic polymorphism of three cultured populations of golden pompano Trachinotus ovatus as revealed by microsatellites |
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| JI Lei,,OU You-jun,LI Jia-er .South China Sea Fisheries Research Institute,CAFS,Guangzhou ,China,.College of Fisheries , Life Science,Shang-hai Ocean University,Shanghai. Genetic polymorphism of three cultured populations of golden pompano Trachinotus ovatus as revealed by microsatellites[J]. Journal of Tropical Oceanography, 2011, 0(3) |
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| Authors: | JI Lei OU You-jun LI Jia-er .South China Sea Fisheries Research Institute CAFS Guangzhou China .College of Fisheries Life Science Shang-hai Ocean University Shanghai |
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| Affiliation: | JI Lei1,2,OU You-jun1,LI Jia-er1 1.South China Sea Fisheries Research Institute,CAFS,Guangzhou 510300,China,2.College of Fisheries and Life Science,Shang-hai Ocean University,Shanghai 201306 |
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| Abstract: | Amplifications with six pairs of microsatellite primers were used to determine the genetic polymorphism of ge-nomic DNA in three cultured populations of golden pompano Trachinotus ovatus,which were collected in Hainan,Shenzheng,and Fujian provinces.The data were analyzed by software package PopGen ver.32,and their phylogenetic trees were con-structed using Mega ver.4.0.The results show that the value of average number of alleles(Na) was 3.67?3.83 in all three populations;the value of average effective numbe... |
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| Keywords: | Trachinotus ovatus cultured population microsatellite DNA genetic diversity |
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