| 哈维氏弧菌生物被膜体外模型的建立及成膜特性研究 |
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| 引用本文： | 李海平,鄢庆枇,徐晓津,苏永全,覃映雪.哈维氏弧菌生物被膜体外模型的建立及成膜特性研究[J].热带海洋学报,2011(3). |
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| 作者姓名： | 李海平 鄢庆枇 徐晓津 苏永全 覃映雪 |
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| 作者单位： | 集美大学水产学院福建省高校水产科技与食品安全重点实验室;厦门大学海洋与环境学院; |
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| 基金项目： | 国家高技术研究发展计划项目(2007AA09Z115) |
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| 摘 要： | 采用改良微孔板法建立哈维氏弧菌Vibrio harveyi TS-628菌株生物被膜体外模型,并进一步研究环境因子对哈维氏弧菌体外形成生物被膜的影响。结果发现,哈维氏弧菌TS-628菌株可以在聚苯乙烯板中形成生物被膜,并且该菌生物被膜的形成受起始菌液浓度、温度、pH等理化因子的显著影响。温度为30℃、NaCl质量分数为3%—6%、pH为7、孵育时间为36h是哈维氏弧菌形成生物被膜的最佳条件。此外哈维氏弧菌在分别经表皮黏液、鳃黏液、肠道黏液、肝脏提取液、脾脏提取液、肌肉提取液包被的基质上成膜效果显著,其中在经肝脏提取液包被的基质上形成的生物被膜量显著高于在其他生物基质上形成的生物被膜量(p<0.01)。
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| 关 键 词： | 哈维氏弧菌 生物被膜 环境因子 微孔板法 生物基质 |
Establishment of in vitro biofilm model and characteristics of biofilm formation of Vibrio harveyi |
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| LI Hai-ping,YAN Qing-pi,XU Xiao-jin,SU Yong-quan,QIN Ying-xue .Fisheries College of Jimei University,Key Laboratory of Science , Technology for Aquaculture , Food Safety,Xiamen ,China,.College of Oceanography , Environmental Science,Xiamen University,Xiamen.Establishment of in vitro biofilm model and characteristics of biofilm formation of Vibrio harveyi[J].Journal of Tropical Oceanography,2011(3). |
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| Authors: | LI Hai-ping YAN Qing-pi XU Xiao-jin SU Yong-quan QIN Ying-xue Fisheries College of Jimei University Key Laboratory of Science Technology for Aquaculture Food Safety Xiamen China College of Oceanography Environmental Science Xiamen University Xiamen |
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| Institution: | LI Hai-ping1,YAN Qing-pi1,XU Xiao-jin1,SU Yong-quan2,QIN Ying-xue1 1.Fisheries College of Jimei University,Key Laboratory of Science and Technology for Aquaculture and Food Safety,Xiamen 361021,China,2.College of Oceanography and Environmental Science,Xiamen University,Xiamen 361005 |
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| Abstract: | Modified microtiter-plate test was used to establish an in vitro biofilm model of Vibrio harveyi TS-628 strain.Effect of environmental factors on bacterial biofilm formation in vitro was also studied.The results show that V.harveyi TS-628 strains could form biofilm in polystyrene plates,and biofilm formation of this bacterium was significantly affected by different environmental factors,such as temperature,initial bacterial concentration,pH,NaCl concentration,and so on.The optimum conditions for biofilm for... |
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| Keywords: | Vibrio harveyi biofilm environmental factor microtiter-plate test biological matrix |
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