黄、渤海小黄鱼耳石元素指纹分析及其在种群补充群体识别中的应用

利用电感耦合等离子质谱ICP-MS元素分析方法,测定了黄、渤海125尾小黄鱼的耳石元素指纹图谱,共检测到Mg、Al、Mn、Cu、Zn、Sr、Ba、Ca等8种指纹元素。不同采样站位小黄鱼耳石元素含量均存在显著性差异,线性判别分析可以有效识别不同站位的小黄鱼群体,判别成功率为65%~96%,整体判别成功率为86%。基于耳石元素指纹图谱特征进行聚类分析,可以将黄、渤海小黄鱼早期补充群体划分为渤海种群、黄海中部种群和南黄海种群,其中黄海中部种群站位交叉明显。

Insights into population structure of juvenile small yellow croaker (Larimichthys polyactis) in the Yellow Sea and the Bohai Sea from otolith elemental fingerprints

Elemental fingerprints were measured on otolith of 125 Larimichthys polyactis juveniles collected from 5 important fishery regions in the Bohai Sea and the Yellow Sea by inductively coupled plasma mass spectrometry (ICP-MS). Otolith elemental figerprints were used as natural tags to discriminate among different sampling regions and the observed site-specific elemental differences in small yellow croaker otoliths suggest a high level of site-fidelity in relation to the 5 fishery regions. Classification accuracy rates from linear discriminate function analyses (LDFA) of otolith chemistry data were high for each region with a mean accuracy of 86%,ranging from 65%-96%,considering every possible combination of elements. Furthermore,cluster analysis of multi-elemental tags suggest that the populations of juvenile fish could be divided into three groups,the Bohai group,the Central Yellow Sea group and the South Yellow Sea group.