Improvement of a Monoclonal Antibody-based Immunoassay for the Determination of Terbutryn Verbesserung eines Immunassays mit monoklonalen Antikörpern zur Bestimmung von Terbutryn

The performance of an existing enzyme immunoassay (EIA) with monoclonal antibodies (mAb) for the determination of terbutryn was improved by the application of a new enzyme tracer. For this purpose the triazine derivative 6-chloro-2-(tert-butylamino)-1,3,5-triazine-4-(6-aminohexane carboxylic acid) was coupled to horseradish peroxidase (HRP). The competitive EIA on microwell plates made it possible to determine terbutryn in the range from 0.05 to 1 μg/L with a 50% B/B₀ value of the test at 0.2 μg/L. The application of the EIA to determine terbutryn in spiked surface waters provided good recoveries of terbutryn without matrix effects.