Fatty acid composition analyses of the DCMU resistant mutants of <Emphasis Type="Italic">Nannochloropsis oculata</Emphasis> (eustigmatophyceae) |
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Authors: | Zhang?Jimin Liu?Shuang Sun?Xue Yang?Guanpin Email author" target="_blank">Zhang?XuechengEmail author Gao?Zhenhui |
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Institution: | (1) North China Sea Monitoring Center, SOA, 22 Fushun Road, 266033 Qingdao, P. R. China;(2) College of Marine Life Sciences, Ocean University of China, 266003 Qingdao, P. R. China |
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Abstract: | Ultraviolet mutagenesis was applied to Nannochloropsis oculata and three mutants resistant to 3-(3, 4-dichlorophenyl)-1,1-dimethylurea (DCMU) were isolated. The cellular chlorophyll a and total lipid content of the wild are higher in the medium supplemented with DCMU than in the control without DCMU. Without
DCMU, the growth rates and chlorophyll a contents of the mutants are similar to those of the wild. Significant changes of fatty acid content and composition have
occurred in DCMU-resistant mutants growing in the medium supplemented with DCMU. The total lipid, palmitic acid (16:0), palmitoleic
acid (16:1ω9) and oleic (18:1ω9) contents decrease significantly, while the vaccenic acid (18:1ω11) increases significantly
and the EPA content of dried powder increases slightly in the mutants. The study may provide a basis to improve EPA content
in Nannochloropsis oculata in the future. |
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Keywords: | Nannochloropsis oculata DCMU Mutation Fatty acid |
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