| Long interspersed elements in three species of Gracilaria （Gracilariaceae, Rhodophyta） |
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| 引用本文： | 李玉晖,姚建亭,高天翔,段德麟. Long interspersed elements in three species of Gracilaria （Gracilariaceae, Rhodophyta）[J]. 中国海洋湖沼学报, 2007, 25(1): 59-66. DOI: 10.1007/s00343-007-0059-z |
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| 作者姓名： | 李玉晖 姚建亭 高天翔 段德麟 |
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| 作者单位： | Department of Aquaculture Ocean University of China Qingdao 266003 China Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China，Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China，Department of Aquaculture Ocean University of China Qingdao 266003 China，Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China |
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| 基金项目： | 国家自然科学基金;中国科学院知识创新工程项目 |
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| 摘 要： | In order to find out whether long interspersed elements (LINEs) existed in macro-algae ge- nomes or not, we tested the LINE homologues in representative families (species): Gracilaria (G. eucheumoides Harv., G. tenuistipitata Chang et Xia, and G. textorii (Sur) De-Toni), Laminaria (L. longis- sima Miyabe and L. japonica Aresch.), and Ulva (U. lactuca L. and U. pertusa Kjellm.) during 2004 to 2005. Polymerase Chain Reaction (PCR) was carried out with degenerate oligonucleotide primers de- signed from LINEs of rice homologues and Cin4 of maize. Cloning and nucleotide sequencing of the PCR products revealed that 4 clones that derived from 3 species of Gracilaria have LINE homologues. The nucleotide sequences of the 4 LINE homologues diverged greatly, but the amino acid sequences deduced from them were relatively conserved. The endonuclease regions of the LINE homologues greatly di- verged from that of other plants, but they had closer phylogenetic relationship to Zepp elements in Chlor- ella sp., which indicated that sequence divergence by vertical transmission has been a major influence on the evolution of algal LINEs.
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| 关 键 词： | 垂直传播 核酸内切酶 寡核苷酸 聚合酶 海水生物化学 |
| 收稿时间： | 2006-05-22 |
| 修稿时间： | 2006-07-24 |
Long interspersed elements in three species of Gracilaria (Gracilariaceae,Rhodophyta) |
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| Li Yuhui,Yao Jianting,Gao Tianxiang,Duan Delin. Long interspersed elements in three species of Gracilaria (Gracilariaceae,Rhodophyta)[J]. Chinese Journal of Oceanology and Limnology, 2007, 25(1): 59-66. DOI: 10.1007/s00343-007-0059-z |
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| Authors: | Li Yuhui Yao Jianting Gao Tianxiang Duan Delin |
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| Affiliation: | (1) Department of Aquaculture, Ocean University of China, Qingdao, 266003, China;(2) Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China;(3) Graduate School of the Chinese Academy of Sciences, Beijing, 100039, China |
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| Abstract: | In order to find out whether long interspersed elements (LINEs) existed in macro-algae ge- nomes or not, we tested the LINE homologues in representative families (species): Gracilaria (G. eucheumoides Harv., G. tenuistipitata Chang et Xia, and G. textorii (Sur) De-Toni), Laminaria (L. longis- sima Miyabe and L. japonica Aresch.), and Ulva (U. lactuca L. and U. pertusa Kjellm.) during 2004 to 2005. Polymerase Chain Reaction (PCR) was carried out with degenerate oligonucleotide primers de- signed from LINEs of rice homologues and Cin4 of maize. Cloning and nucleotide sequencing of the PCR products revealed that 4 clones that derived from 3 species of Gracilaria have LINE homologues. The nucleotide sequences of the 4 LINE homologues diverged greatly, but the amino acid sequences deduced from them were relatively conserved. The endonuclease regions of the LINE homologues greatly di- verged from that of other plants, but they had closer phylogenetic relationship to Zepp elements in Chlor- ella sp., which indicated that sequence divergence by vertical transmission has been a major influence on the evolution of algal LINEs. |
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| Keywords: | Endonuclease gene long interspersed element (LINE) phylogeny Gracilaria Gracilariaceae Rhodophyta |
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