Metamorphosis of Magnetospirillum magneticum AMB-1 cells |
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Authors: | Fengli Zhang Kui Yu-Zhang Sanjun Zhao Tian Xiao Michel Denis Longfei Wu |
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Affiliation: | 1. Laboratoire de Chimie Bactérienne, UPR9043, Institut de Biologie Structurale et Microbiologie, CNRS, Marseille, France 4. Laboratory of Marine Biotechnology, School of Life Sciences & Biotechnology, Key Laboratory of Microbial Metabolism, Ministry of Education, Shanghai Jiao Tong University, Shanghai, 200240, China 2. Laboratoire de Microscopies et d’Etude de Nanostructures (LMEN), Université de Reims, Champagne-Ardenne, Reims, France 5. Key Laboratory of Marine Ecology & Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China 3. Laboratoire de Microbiologie, Géochimie et Ecologie Marines, CNRS UMR6117, Centre d’Océanologie de Marseille, Université de la Méditerranée, 163 avenue de Luminy, Case 901, 13 288, Marseille cedex 9, France
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Abstract: | Magnetospirillum magneticum strain AMB-1 belongs to the family of magnetotactic bacteria. It possesses a magnetosome chain aligning, with the assistance of cytoskeleton filaments MamK, along the long axis of the spiral cells. Most fresh M. magneticum AMB-1 cells exhibit spiral morphology. In addition, other cell shapes such as curved and spherical were also observed in this organism. Interestingly, the spherical cell shape increased steadily with prolonged incubation time. As the actin-like cytoskeleton protein MreB is involved in maintenance of cell shapes in rod-shaped bacteria such as Escherichia coli and Bacillus subtilis, the correlation between MreB protein levels and cell shape was investigated in this study. Immunoblotting analysis showed that the quantity of MreB decreased when the cell shape changed along with incubation time. As an internal control, the quantity of MamA was not obviously changed under the same conditions. Cell shape directs cell-wall synthesis during growth and division. MreB is required for maintaining the cell shape. Thus, MreB might play an essential role in maintaining the spiral shape of M. magneticum AMB-1 cells. |
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