Effect of carbon source on growth and lipid accumulation in <Emphasis Type="Italic">Chlorella sorokiniana</Emphasis> GXNN01 |
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Authors: | Hongjin Qiao Guangce Wang |
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Institution: | (1) College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan Road, 510640 Guangzhou, China;(2) Department of Biological Sciences and Biotechnology, Tsinghua University, 100084 Beijing, China;(3) William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, USA; |
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Abstract: | Heterotrophic culture of microalgae to develop methods of increasing biomass productivity and storage lipids has brought new
insight to commercial biodiesel production. To understand the relationship between heterotrophy and lipid production, the
effects of carbon sources on the growth and lipid accumulation of Chlorella sorokiniana GXNN01 was studied. The alga exhibited an increased growth rate in response to the addition of carbon sources, which reached
the stationary phase after 48 h at 30°C. In addition, glucose and NaAc had a significant effect on the lipid accumulation
during the early-stationary phase. Specifically, the lipid content was 0.237±0.026 g g−1 cell dry weight and 0.272±0.041 g L−1 when glucose was used as the carbon source, whereas the lipid content reached 0.287±0.018 g g−1 cell dry weight and 0.288±0.008 g L−1 when NaAc was used as the carbon source. The neutral lipid content was found to first decrease and then increase over time
during the growth phase. A glucose concentration of 20 mmol L−1 gave the maximal lipid yield and the optimum harvest time was the early-stationary phase. |
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