海洋褐藻羊栖菜(Sargassum fusiforme)中马尾藻甾醇、岩藻甾醇的分类纯化及抗菌、抗氧化活性研究

本文评价海洋褐藻羊栖菜(Sargassum fusiforme)中分离纯化的总甾醇、马尾藻甾醇(Saringosterol)和岩藻甾醇(Fucosterol)抗菌和抗氧化活性。马尾藻甾醇和岩藻甾醇化合物采用红外光谱、核磁共振光谱和高分辨质谱确定其结构。实验结果显示,总甾醇含量在30、60和80mg/m L对金黄色葡萄球菌(Staphylococcus aureus)和变形链球菌(S.mutans)具有抑制作用。马尾藻甾醇和岩藻甾醇对金黄色葡萄球菌和变形链球菌具有高活性,最小抑制浓度范围为4—32mg/m L。总甾醇还显示出对大肠杆菌(Escherichia coli)菌株1924和1356的抗菌作用。而马尾藻甾醇和岩藻甾醇在4—32mg/m L浓度范围内体外对大肠杆菌的抗菌作用不明显。马尾藻甾醇和岩藻甾醇对耐药性革兰氏阳性临床分离菌株具有显著的抑菌活性。另外,药理实验结果显示,总甾醇、马尾藻甾醇和岩藻甾醇的抗氧化活性与它们清除DPPH、ABTS、超氧化物和羟基自由基的能力有关。

SARINGOSTEROL AND FUCOSTEROL, TWO STEROLS FROM SARGASSUM FUSIFORME WITH ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES

In the present study, we evaluated the antibacterial and antioxidant properties of saringosterol and fucosterol extracted from Sargassum fusiforme against Gram-positive and Gram-negative bacteria using DPPH radical scavenging assay, ABTS^·+ radical decolorization assay, superoxide radical assay and hydroxyl radical assay. Total sterols showed inhibitory effects against Staphylococcus aureus and Streptococcus mutans at 30 and 60mg/mL. Saringosterol and fucosterol were highly active against S. aureus and S. mutans with minimum inhibitory concentrations ranging 4-32mg/mL. Total sterols also displayed antibacterial effects against Escherichia coli strains 1924 and 1356. However, saringosterol and fucosterol did not exhibit antibacterial effects against E. coli at 4-32mg/mL in vitro. Additionally, saringosterol and fucosterol were highly active against multidrug-resistant Gram-positive clinical isolates. The antioxidant activities of total sterols, saringosterol and fucosterol were related to their ability to scavenge DPPH, ABTS, superoxide and hydroxyl radicals, indicated that the antioxidant activities of the total sterols, saringosterol and fucosterol were related to their ability to scavenge DPPH radical, ABTS radical, superoxide radical, and hydroxyl radical.