饲料添加多维对欧洲鳗鲡(Anguilla anguilla)生长性能、消化酶活性和免疫的影响

采用单因子试验设计方法,研究了饲料中添加多维对欧洲鳗鲡(Anguilla anguilla)生长性能、消化酶活性和免疫的影响。试验用鳗(5.93±0.02)g,分6组(每组3重复,每个重复30尾鱼),分别投喂添加不同浓度(0%,0.25%,0.50%,0.75%,1.00%,1.25%)的多维饲料42d。结果表明,随着多维添加水平的上升,各组鳗鲡增重率和特定生长率呈先升高后降低趋势,且差异显著(P0.05),当多维浓度为0.75%时增重率达最高峰值。鳗鲡的肝体指数和肥满度受多维影响较小,在各组间均无显著差异(P0.05)。饲料中添加0.75%浓度的多维显著降低了鱼体水分含量,使鱼体粗蛋白和灰分含量得到显著提高(P0.05),但各试验组鳗鲡粗脂肪含量并无显著差异(P0.05)。相比对照组,各添加组鳗鲡肠道胰蛋白酶、淀粉酶、脂肪酶活性及血清超氧化物歧化酶、溶菌酶和碱性磷酸酶活性均得到显著提高(P0.05),且0.75%组鳗鲡血清总蛋白浓度达到最大(P0.05)。综合本研究结果可知多维在饲料中的适宜添加水平为0.75%。

EFFECTS OF THE DIFFERENT LEVELS OF DIETARY MULTIVITAMIN ON GROWTH PERFORMANCE, ACTIVITIES OF DIGESTIVE ENZYMES AND IMMUNE RESPONSE OF ANGUILLA ANGUILLA

A single-factor experiment was conducted to study the effects of dietary multivitamin on growth performance, activities of digestive enzymes and immune response of Anguilla anguilla. A. anguilla juveniles [body weight (5.93 ± 0.02)g] were randomly divided into 6 treatments (with 3 replicates each, 30 fishes for each replicate). Six experimental diets were prepared (adding 0%, 0.25%, 0.5%, 0.75%, 1.0%, 1.25% multivitamin), respectively to feed anguilla for 42d. The results showed that with increase of dietary multivitamin level, eel weight gain ratio and the specific growth ratio in each group showed a trend of first rising then falling, and got to a significant difference finally (P < 0.05). While the concentration of multivitamin was 0.75%, weight gain ratio had reached the highest peak. Hepatosomatic index and condition factor had no apparent change among the groups. At inclusion of 0.75% level, the body moisture decreased notably (P < 0.05), and the crude protein and the ashincreased significantly (P < 0.05), but no significant differences among the crude lipid content (P > 0.05), compared with the control group. Based on the results of the trial above, the recornmented of dose of multivitamin in diets of A. anguilla is 0.75%.