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Cytochrome P450 isozyme protein verified in the skin of southern hemisphere humpback whales (Megaptera novaeangliae): implications for biochemical biomarker assessment |
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| Authors: | Waugh Courtney A Huston Wilhelmina M Noad Michael J Bengtson Nash Susan |
| Affiliation: | a The University of Queensland, The National Research Centre for Environmental Toxicology, Brisbane, QLD 4108, Australia b Queensland University of Technology, Institute of Health and Biomedical Innovation, Kelvin Grove, QLD 4059, Australia c The University of Queensland, Cetacean Ecology and Acoustics Lab (CEAL), School of Veterinary Science, Gatton, QLD 4343, Australia d Griffith University, Atmospheric Environment Research Centre (AERC), Nathan, QLD 4111, Australia |
| Abstract: | Large mysticete whales represent a unique challenge for chemical risk assessment. Few epidemiological investigations are possible due to the low incidence of adult stranding events. Similarly their often extreme life-history adaptations of prolonged migration and fasting challenge exposure assumptions. Molecular biomarkers offer the potential to complement information yielded through tissue chemical analysis, as well as providing evidence of a molecular response to chemical exposure. In this study we confirm the presence of cytochrome P450 reductase (CPR) and cytochrome P450 isoenzyme 1A1 (CYP1A1) in epidermal tissue of southern hemisphere humpback whales (Megaptera novaeangliae). The detection of CYP1A1 in the integument of the humpback whale affords the opportunity for further quantitative non-destructive investigations of enzyme activity as a function of chemical stress. |
| Keywords: | Humpback whale CYP1A1 Biomarkers Western blot |
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