Morphological changes in branchial mitochondria-rich cells of the teleost Paralichthys olivaceus as a potential indicator of CO2 impacts |
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Authors: | Masahiro Hayashi Takashi Kikkawa Atsushi Ishimatsu |
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Institution: | 1. Institute for East China Sea Research, Nagasaki University, 1551-7 Tairamachi, Nagasaki 851-2213, Japan;2. Central Laboratory, Marine Ecology Research Institute, Onjuku, Chiba 299-5105, Japan |
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Abstract: | We studied the morphological and biochemical changes of mitochondria-rich cells (MRCs) of a demersal teleost, Paralichthys olivaceus, during exposure to 0.98, 2.97 and 4.95 kPa pCO2. The apical opening area of MRCs increased 2.2 and 4.1 times by 24 h exposure to 2.97 and 4.95 kPa pCO2, respectively, while the cross-sectional area or density of MRCs did not change. Gill Na+/K+-ATPase activity more than doubled at 72 h and then returned to the pre-exposure level at 168 h in 0.98 kPa pCO2, while it increased 1.7 times at 24 h at 4.95 kPa. These results indicate that the apical opening area of MRCs and the gill Na+/K+-ATPase activity may be used as an indicator of acute (up to 72 h), but not chronic, impacts of high (>1 kPa) seawater CO2 conditions in P. olivacues. Limitations of those parameters as indices of CO2 impacts are discussed. |
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Keywords: | Carbon dioxide CO2 leakage Environmental hypercapnia Mitochondria-rich cell Na+/K+-ATPase activity Paralichthys olivaceus |
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