| 利用酒精酵母细胞表面展示技术制备活疫苗及其潜在应用 |
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| 引用本文： | 池振明,高玲美,岳礼溪,朱开玲,李静.利用酒精酵母细胞表面展示技术制备活疫苗及其潜在应用[J].中国海洋大学学报(自然科学版),2007,37(6):947-950,946. |
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| 作者姓名： | 池振明 高玲美 岳礼溪 朱开玲 李静 |
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| 作者单位： | 中国海洋大学海洋生命学院,山东,青岛,266003 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 最近几年，出现把有关的基因在宿主表达之后的目的蛋白分泌并固定在细菌、病毒和酵母菌细胞表面上的技术一表面展示技术。在这些宿主中，酒精酵母菌是最佳的微生物。本文对利用酒精酵母细胞表面展示技术制备活疫苗的基本原理，在酵母细胞表面展示蛋白质的优点，以及哈维氏弧菌溶血素蛋白在酵母菌细胞表面上展示及其作为活疫苗的潜在应用作了综述。研究结果表明在酒精酵母菌细胞表面上展示的哈维氏弧菌溶血素蛋白在海洋动物中具有很好的免疫原性和免疫保护性。
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| 关 键 词： | 酒精酵母 细胞表面展示 活疫苗 哈维氏弧菌溶血素蛋白 |
| 文章编号： | 1672-5174（2007）06-947-05 |
| 收稿时间： | 2007-05-18 |
| 修稿时间： | 2007-05-28 |
Preparation of Live Vaccine with Saccharomyces cerevisiae by Using Surface-Display Technique and Its Potential Applications |
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| CHI Zhen-Ming,GAO Ling-Mei,YUE Li-Xi,ZHU Kai-Ling,LI Jing.Preparation of Live Vaccine with Saccharomyces cerevisiae by Using Surface-Display Technique and Its Potential Applications[J].Periodical of Ocean University of China,2007,37(6):947-950,946. |
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| Authors: | CHI Zhen-Ming GAO Ling-Mei YUE Li-Xi ZHU Kai-Ling LI Jing |
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| Abstract: | In recent years, a surface-display technique has received increasing attention. Among the hosts,Saccharomyces cerevisiae is the best microorganism. In this paper, the principle of preparation of live vaccine with Saccharomyces cerevisiae by using the surface-display technique , the advantage of displaying protein on surface of the yeast cell, and surface-display of haemolysin from Vibrio harveyi on the yeast cells and their potential applications as live vaccine in marine fish are reviewed. It was found that displayed haemolysin on the
yeast cells is immunoantigenic and immunoprotective in marine fish. |
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| Keywords: | Saccharomyces cerevisiae surface-display live vaccine haemolysin of Vibrio harveyi |
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