Development of an in situ loop-mediated isothermal amplification technique for chromosomal localization of DNA sequences |
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| Authors: | MENG Qinglei WANG Shi ZHANG Lingling HUANG Xiaoting BAO Zhenmin |
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| Affiliation: | 1. Key Laboratory of Marine Genetics and Breeding(MGB), Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China;Shandong Provincial Key Laboratory of Freshwater Genetics and Breeding, Freshwater Fisheries Research Institute of Shandong Province, Jinan 250117, China
2. Key Laboratory of Marine Genetics and Breeding(MGB), Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China |
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| Abstract: | In situ loop-mediated isothermal amplification (in situ LAMP) combines in situ hybridization and loop-mediated isothermal amplification (LAMP) techniques for chromosomal localization of DNA sequences. In situ LAMP is a method that is generally more specific and sensitive than conventional techniques such as fluorescence in situ hybridization (FISH), primed in situ labeling (PRINS), and cycling primed in situ labeling (C-PRINS). Here, we describe the development and application of in situ LAMP to identify the chromosomal localization of DNA sequences. To benchmark this technique, we successfully applied this technique to localize the major ribosomal RNA gene on the chromosomes of the Zhikong scallop (Chlamys farreri). |
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| Keywords: | chromosomal localization in situ loop-mediated isothermal amplification (in situ LAMP) major rRNA Chlamys farreri |
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