| 亚临界流体中酶催化制备富含多不饱和脂肪酸甘油酯 |
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| 引用本文： | 于刚,徐伟,栾东磊,孙兆敏,薛勇,薛长湖.亚临界流体中酶催化制备富含多不饱和脂肪酸甘油酯[J].中国海洋大学学报(自然科学版),2009,39(2). |
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| 作者姓名： | 于刚 徐伟 栾东磊 孙兆敏 薛勇 薛长湖 |
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| 作者单位： | 1. 中国水产科学研究院南海水产研究所,广东,广州,510300;中国海洋大学食品科学与工程学院,山东,青岛,266003
2. 中国海洋大学食品科学与工程学院,山东,青岛,266003 |
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| 基金项目： | 国家高技术研发展规划项目，中央级公益性科研院所基本科研业务费专项基金 |
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| 摘 要： | 研究亚临界流体中酶催化制备富含多不饱和脂肪酸甘油酯.采用亚临界R134a作为固定化脂肪酶Nonozym435催化富含n-3多不饱和脂肪酸甘油酯合成的反应介质,并利用响应面法对影响甘油酯合成的工艺条件进行了优化.结果表明,反应时间、酶用量、底物摩尔比、初始水分是影响酯化率的显著因素.通过对转化率的拟合模型响应面分析,得到了优化工艺条件:在亚临界R134a体系中,体系压力4 MPa,温度40 ℃,反应时间3.59 h,酶量:底物量的3.44%,底物摩尔比质量分数为4.55∶1(甘油/游离脂肪酸),初始水分含量0.81%;在此优化工艺条件下,酯化率的预测值为78.89%,试验值为84.06%.试验值与预测值的相符性很好,RSM模型的有效性得到验证.
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| 关 键 词： | 亚临界R134a 酶催化 多不饱和脂肪酸甘油酯 响应面 |
Lipase-Catalyzed Preparation of n-3 Polyunsaturated Fatty Acid Glyceride in a Subcritical Fluid |
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| YU Gang,XU Wei,LUAN Dong-Lei,SUN Zhao-Min,XUE Yong,XUE Chang-Hu.Lipase-Catalyzed Preparation of n-3 Polyunsaturated Fatty Acid Glyceride in a Subcritical Fluid[J].Periodical of Ocean University of China,2009,39(2). |
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| Authors: | YU Gang XU Wei LUAN Dong-Lei SUN Zhao-Min XUE Yong XUE Chang-Hu |
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| Institution: | 1. South China Sea Fisheries Research Institute;Chinese Academy of Fishery Sciences;Guangzhou 510300;China;2. College of Food Science and Engineering;Ocean University of China;Qingdao 266003;China |
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| Abstract: | Lipase-catalyzed selective esterification of glycerol with n-3 polyunsaturated fatty acid from fish oil to produce n-3 polyunsaturated fatty acid glyceride was performed in subcritical R134a. Response surface methodology was used to model the reaction. Commercial immobilized lipase from Candida antarctica(Novozym 435) was the biocatalyst for the reaction. The reaction factors investigated were reaction time, water content, substrate molar ratio, and enzyme amount. A well-fitting quadratic polynomial regress... |
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| Keywords: | subcritical R134a lipase-catalyzed n-3 polyunsaturated fatty acid glyceride response surface methodology |
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