Phylogenetic analysis and biological characteristic tests of marine bacteria isolated from Southern Ocean (Indian sector) water |
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| Authors: | GUPTA Pratibh BALAJI Raju PARANI M CHANDRA T S SHUKLA P KUMAR Anil BANDOPADHYAY Rajib |
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| Affiliation: | Department of Bio-Engineering, Birla Institute of Technology, Mesra, Ranchi 835215, Jharkhand, India,Department of Genetic Engineering, Kattankulathur Campus, SRM University, Chennai 600033, Tamilnadu, India,Department of Genetic Engineering, Kattankulathur Campus, SRM University, Chennai 600033, Tamilnadu, India,Microbiology and Genetics Laboratory, Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600036, Tamilnadu, India,Department of Bio-Engineering, Birla Institute of Technology, Mesra, Ranchi 835215, Jharkhand, India;Department of Microbiology, Maharshi Dayanand University, Rohtak 124001, Haryana, India,OSSG Department, National Centre for Antarctic and Ocean Research, Ministry of Earth Sciences, Government of India, Headland Sada, Vasco-da-Gama, Goa 403804, India and Department of Bio-Engineering, Birla Institute of Technology, Mesra, Ranchi 835215, Jharkhand, India;UGC Centre of Advanced Study, Department of Botany, the University of Burdwan, Golapbag, Bardhaman 713104, West Bengal, India |
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| Abstract: | Fifty-seven bacteria were isolated from Southern Ocean (Indian sector) water samples which were collected from different latitude and longitude of the ocean. All the isolates were able to grow at 4℃, 20℃, 37℃ and tolerable NaCl concentration up to 13.5% (w/v). 29 out of 57 isolates were identified using 16S rDNA amplification and the sequences were submitted to National Center for Biotechnology Information (NCBI). All the isolates were classified by using Ribosomal Database Project (RDP) and found that isolates belongs to Proteobacteria and Bacteriodes. The average G+C content was 56.4%. The isolates were screened for the presence of extracellular enzymes, viz. amylase, catalase, urease, esterase, lipase and protease. The disc diffusion method is used to screen antibiotic production by the isolates against four pathogenic bacteria, viz. Salmonella typhimurium (NCIM 2501), Staphylococcus aureus (NCIM 2122), Bacillus subtilis (NCIM 2193), and Pseudomonas aeruginosa (NCIM 2036). Nine out of 29 were found to be antibiotic producer. |
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| Keywords: | Southern Ocean marine bacteria 16S rDNA phylogenetic tree antibacterial |
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