|
|||||
|
|
| Identification of Phase and Sex-related ISSR Markers of Red Alga Gracilaria lemaneiformis | |
| 作者姓名： | SUN Xue ZHANG Xuecheng MAO Yunxiang SUI Zhenghong QIN Song |
| 作者单位： | [1]College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China [2]Key Laboratory of Marine Biotechnology , Ningbo University, Ningbo 315211, P. R. China [3]Institute of Oceanology, Chinese Academy of Scienees, Qingdao 266071, P.R. China |
| 摘 要： | Six ISSR primers are employed to display the polymorphism of different phases and sexes of red alga Gracilaria lemaneiformis, and two of them, P1 and P3, amplified distinct band patterns. The ISSR pattern amplified by primer P1 of the female gametophyte is identical to that of tetrasporophyte, but distinct from that of male gametophyte. Of the bands produced by primer P3, one is specific to female gametophyte. Three morphologically similar fronds can be easily identified using ISSR technique. Two specific markers, SM1 and SF3, related to male gametophyte and female gametophyte, are cloned and sequenced. The homologous sequences of SM1 are found to encode a hypothetical protein. There is no homologous sequence of SF3 that can be found in GenBank. |
| 关 键 词： | 相位 性别 多态现象 配偶体 水生生物 |
Identification of phase and sex-related ISSR markers of red algaGracilaria lemaneiformis |
|
| SUN Xue ZHANG Xuecheng MAO Yunxiang SUI Zhenghong QIN Song.Identification of Phase and Sex-related ISSR Markers of Red Alga Gracilaria lemaneiformis[J].Journal of Ocean University of China,2006,5(1):82-84. | |
| Authors: | Sun Xue Zhang Xuecheng Mao Yunxiang Sui Zhenghong Qin Song |
| Institution: | 1. College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P .R .China;Key Laboratory of Marine Biotechnology, Ningbo University, Ningbo 315211, P .R .China 2. College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P .R .China 3. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, P .R .China |
| Abstract: | Six ISSR primers are employed to display the polymorphism of different phases and sexes of red alga Gracilaria lemaneiformis, and two of them, P1 and P3, amplified distinct band patterns. The ISSR pattern amplified by primer P1 of the female gametophyte is identical to that of tetrasporophyte, but distinct from that of male gametophyte. Of the bands produced by primer P3, one is specific to female gametophyte. Three morphologically similar fronds can be easily identified using ISSR technique. Two specific markers, SM1 andSF3, related to male gametophyte and female gametophyte, are cloned and sequenced. The homologous sequences of SM1 are found to encode a hypothetical protein. There is no homologous sequence of SF3 that can be found in GenBank. |
| Keywords: | Gracilaria lemaneiformis phase sex polymorphism ISSR |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ | |