| 长江中华鲟遗传多样性变化 |
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| 引用本文： | 曾 勇,危起伟,汪登强.长江中华鲟遗传多样性变化[J].海洋科学,2007,31(10):67-69. |
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| 作者姓名： | 曾 勇 危起伟 汪登强 |
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| 作者单位： | 中国水产科学研究院,长江水产研究所,湖北,荆州,434000 |
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| 基金项目： | 国家科学技术部专项基金
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中国水产科学院科研项目 |
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| 摘 要： | 采用随机扩增多态性DNA(RAPD)技术对2000年共30尾长江中华鲟(Acipenser sinensis)幼鲟遗传多样性进行了分析。共用40个10bp长的随机引物,在25个可分析的引物中,只有OPK01,OPK02,OPK03,OPK09和OPK14RAPD-PCR产物有多态现象,多态引物占20%。25个引物共扩增出101条稳定的DNA带。其中12条为多态带,多态座位比例为9.9%。香农遗传多样性指数(H0)为2.6332,遗传多样性指数(H)为0.0261,遗传相似性指数平均为0.9824,遗传距离平均为0.0176。其遗传多样性明显低于葛洲坝截流以前。
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| 关 键 词： | 中华鲟(Acipenser sinensis) 随机扩增多态性DNA(RAPD) 遗传多样性 |
| 文章编号： | 1000-3096（2007）10-0067-03 |
| 收稿时间： | 2004/7/30 0:00:00 |
| 修稿时间： | 2004-07-302006-05-15 |
The variation of genetic diversity of Chinese sturgeon (Acipenser sinensis) in the Yangtz River |
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| ZENG Yong,WEI Qi-wei,WANG Deng-qiang.The variation of genetic diversity of Chinese sturgeon (Acipenser sinensis) in the Yangtz River[J].Marine Sciences,2007,31(10):67-69. |
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| Authors: | ZENG Yong WEI Qi-wei WANG Deng-qiang |
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| Institution: | Yangtz River Fisheries Research Institute, the Chinese Academy of Fishery Science, Jingzhou 434000,China |
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| Abstract: | Totally 30 samples collected in 2000 were analyzed by random amplification of polymorphic DNA(RAPD).40 decamer primers were applied for analysis and only 25 primers gave stable results.Among 25 primers studied only 5 primers(20%)such as OPK01,OPK02,OPK03,OPK09 and OPK14 produced polymorphic bands.Total 101 bands(locus)were obtained using 25 primers.Out of the 101 bands(locus),10 bands were polymorphic.The percentage of polymorphic loci was about 9.9%.The Shannon's index of phenotypic diversity H0=2.633 2.The genetic diversity H=0.026 1.Genetic similarity and genetic distance between individuals were 0.982 4,0.017 6 respectively.The genetic diversity of the samples in this study was lower than that before the construction of Gezhouba dam. |
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| Keywords: | Chinese sturgeon(Acipensert sinensis) random amplification of polymorphic DNA(RAPD) genetic diversity |
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