Modulation of p53 gene expression in the intertidal copepod Tigriopus japonicus exposed to alkylphenols |
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| Institution: | 1. SLCR-Sea Lice Research Center, Institute of Marine Research, 5817 Bergen, Norway;2. SLCR-Sea Lice Research Center, Department of Biology, University of Bergen, Thormøhlensgt. 55, 5008 Bergen, Norway;1. Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 440-746, South Korea;2. Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 133-791, South Korea;3. Division of Life Sciences, Korea Polar Research Institute, Korea Institute of Ocean Science and Technology, Incheon 406-840, South Korea;4. Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 133-791, South Korea;5. Department of Marine Science, College of Natural Sciences, Incheon National University, Incheon 406-772, South Korea;1. Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 440-746, South Korea;2. Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 133-791, South Korea;3. Department of Marine Science, College of Natural Sciences, Incheon National University, Incheon 406-772, South Korea |
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| Abstract: | The copepod, Tigriopus japonicus has been recognized as an excellent marine model species for ecotoxicological studies. The present study cloned and characterized the p53 gene of this copepod and studied its expression pattern. We discovered that p53 expression patterns varied among different developmental stages of the copepod, having the highest expression in the adult. Such variation was possibly associated with the molting cycle. By using real-time RT-PCR, we further investigated the modulatory pattern of the p53 gene in the copepod after exposure to three alkylphenols (i.e. nonylphenol, octylphenol, and bisphenol A) which are known as endocrine disruption chemicals (EDCs). The results showed that the three alkylphenols significantly induced p53 gene expression in the copepod, indicating the involvement of p53 in such stress-responses. Thus, the copepod p53 gene provides one of the stress-response biomarkers for exposure to EDC-like compounds. |
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