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美国红鱼(Sciaenops ocellata)的核型、Ag-NORs和 C-带分析
引用本文:王晓艳,王世锋,张建设,吴常文. 美国红鱼(Sciaenops ocellata)的核型、Ag-NORs和 C-带分析[J]. 海洋与湖沼, 2012, 43(1): 84-87
作者姓名:王晓艳  王世锋  张建设  吴常文
作者单位:1. 浙江海洋学院海洋科学学院国家海洋设施养殖工程技术研究中心,舟山,316004
2. 海南大学热带生物资源教育部重点实验室,海口,570228
基金项目:国家科技支撑计划项目, 2011BAD13B08 号; 国家自然科学基金项目, 31060355 号; 海洋渔业科学与技术浙江省重中之重学科开放课题资助项目, 20100115 号
摘    要:采用常规核型分析、Ag-NORs及C-带技术对美国红鱼的核型特征进行了研究。结果表明,美国红鱼二倍体染色体数目为48,48条染色体均为端部着丝粒染色体,核型公式为2n=48t,NF=48;核仁组织区位于第1对同源染色体靠近着丝粒的位置;美国红鱼所有染色体的着丝粒位置显示为C-带阳性,同时发现核仁组织区也表现为异染色质;第1对染色体表现为长度多态性,与核仁组织区多态性相关,而非性染色体。

关 键 词:美国红鱼  核型  Ag-NORs  C-带
收稿时间:2010-10-19
修稿时间:2010-12-26

THE KARYOTYPE, Ag-NORs AND C-BANDING OF SCIAENOPS OCELLATA
WANG Xiao-Yan,WANG Shi-Feng,ZHANG Jian-She and WU Chang-Wen. THE KARYOTYPE, Ag-NORs AND C-BANDING OF SCIAENOPS OCELLATA[J]. Oceanologia Et Limnologia Sinica, 2012, 43(1): 84-87
Authors:WANG Xiao-Yan  WANG Shi-Feng  ZHANG Jian-She  WU Chang-Wen
Affiliation:Marine Science College of Zhejiang Ocean University, National Engineering Research Center of Marine Facilities Aquaculture;Hainan University, Key Laboratory of Tropical Aquatic Biotechnology of Education Ministry;Marine Science College of Zhejiang Ocean University, National Engineering Research Center of Marine Facilities Aquaculture;Marine Science College of Zhejiang Ocean University, National Engineering Research Center of Marine Facilities Aquaculture
Abstract:The karyotype characteristics of Sciaenops ocellata was investigated using conventional staining, Ag-NORs and C-banding. The results are as follows: S. ocellata had a diploid chromosome number of 48 and its karyotype formula was 48t, NF=48; The Ag-NORs appeared in chromosome 1; The centromere of all chromosomes and the Ag-NORs presented positive C-banding; The length polymorphism of Chromosome 1 was not sex related but co-occurrenced with the Ag-NORs.
Keywords:Sciaenops ocellata   Karyotype   Ag-NORs   C-banding
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