| 杜氏藻与大肠杆菌跨界融合初探 |
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| 引用本文： | 刘广发,楼士林,李庆顺,赖瑞卿. 杜氏藻与大肠杆菌跨界融合初探[J]. 海洋科学, 1993, 17(4): 35-40 |
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| 作者姓名： | 刘广发 楼士林 李庆顺 赖瑞卿 |
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| 作者单位： | 夏门大学生物学系 361005(刘广发，楼士林，李庆顺)，夏门大学生物学系 361005(赖瑞卿) |
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| 摘 要： | 以链霉蛋白酶制得了杜氏藻(嗜盐单胞绿藻)的原生质体。采用聚乙二醇(PEG)-钙离子法将它们与大肠杆菌(含氯霉素乙酰转移酶基因质粒)的球1,20,1状体进行融合。“融合子”酷似杜氏藻。它们对氯霉素抗性的显著提高寓示了大肠杆菌的质粒已转移到“融合子”细胞中;而且,“融合子”的蛋白质组分与杜氏藻和大肠杆菌相比已发生了较明显的变化。
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| 关 键 词： | 杜氏藻 大肠杆菌 融合 |
| 收稿时间： | 1991-10-14 |
A RESEARCH ON INTERKINGDOM PROTOPLAST FUSION BETWEEN GREEN ALGA DUNALIELLA AND E. COLl |
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| Liu Guangfa,Lou Shilin,Li Qingshun and Lai Ruiqing. A RESEARCH ON INTERKINGDOM PROTOPLAST FUSION BETWEEN GREEN ALGA DUNALIELLA AND E. COLl[J]. Marine Sciences, 1993, 17(4): 35-40 |
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| Authors: | Liu Guangfa Lou Shilin Li Qingshun Lai Ruiqing |
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| Abstract: | True protoplasts of the halophilic green alga Dunaliella peircei proven by electron micrograph were induced by treatment with pronase. They were fused with the spheroplasts of E. coli harboring P35S-CAT plasmids by polyethylene glycol(PEG) and calcium cations and then allowed to regenerate on selection media. Some Dunaliella-like clones were isolated from the media with acquired resistance to chloramphenicol appears to be the result of a genetic transfer from the plasmids harbored in E. coli. Moreover the composition of proteins extracted from the fusant were fairly different from either D. peircei or E. coli. |
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| Keywords: | Dunaliella peircei E.coli Fusion |
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