Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease |
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Authors: | Zhaolan Mo Dongsheng Guo Yunxiang Mao Xuhong Ye Yuxia Zou Peng Xiao Bin Hao |
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Affiliation: | (1) Department of Marine Biology, College of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao, 266003, PRC; |
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Abstract: | We cloned and sequenced a prtV-like gene from Vibrio anguillarum M3 strain. This prtV gene encodes a putative protein of 918 amino acids, and is highly homologous to the V. cholerae prtV gene. We found that a prtV insertion mutant strain displayed lower gelatinase activity on gelatin agar, lower protease activity against azocasein, and lower activity for four glycosidases. This prtV mutant strain also had increased activity for two esterases in its extracellular products, as analyzed by the API ZYM system. In addition, the prtV mutant strain exhibited decreased growth in turbot intestinal mucus and reduced hemolytic activity on turbot erythrocytes. Infection experiments showed that the LD50 of the prtV mutant strain increased by at least 1 log compared to the wild-type in turbot fish. We propose that prtV plays an important role in the pathogenesis of V. anguillarum. |
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