| Purification and characterization of a new thermostable κ-carrageenase from the marine bacterium Pseudoalteromonas sp. QY203 |
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| 作者姓名： | LI Shangyong JIA Panpan WANG Linna YU Wengong HAN Feng |
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| 作者单位： | Key Laboratory of Marine Drugs, Chinese Ministry of Education;Provincial Key Laboratory of Glycoscience and Glycotechnology of Shandong Province and School of Medicine and Pharmacy, Ocean University of China |
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| 基金项目： | National Science Foundation of China，Program for Changjiang Scholars and Innovative Research Team in University，Special Fund for Marine Scientific Research in the Public Interest，the Fundamental Research Funds for the Central Universities |
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| 摘 要： | A new extracellular κ-carrageenase, namely CgkP, 34.0 kDa in molecular weight, was purified from Pseudoalteromonas sp. QY203. CgkP showed relatively high activity at acidities ranging from pH6.0 to pH9.0 and temperatures ranging from 30℃ to 50℃ with the highest activity at 45℃ and pH7.2. Sodium chloride increased its activity markedly, and KCl increased its activity slightly. The divalent and trivalent metal ions including Cu2+ , Ni2+ , Zn2+ , Mn2+ , Al3+ and Fe3+ significantly inhibited its activity, while Mg2+ did not. CgkP remained 70% of original activity after being incubated at 40℃ for 48 h, and remained 80% of the activity after being incubated at 45℃ for 1 h. It exhibited endo-κ-carrageenase activity, mainly depolymerizing the κ-carrageenan into disaccharide and tetrasaccharide. CgkP was more thermostable than most of previously reported κ-carrageenases with a potential of being used in industry.
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| 关 键 词： | κ-carrageenase purification characterization thermostability Pseudoalteromonas |
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